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Multifunctional human CD56 low CD16 low natural killer cells are the prominent subset in bone marrow of both healthy pediatric donors and leukemic patients.
- Source :
-
Haematologica [Haematologica] 2015 Apr; Vol. 100 (4), pp. 489-98. Date of Electronic Publication: 2015 Jan 16. - Publication Year :
- 2015
-
Abstract
- We phenotypically and functionally characterized a distinct CD56(low) natural killer cell subset based on CD16 expression levels in bone marrow and peripheral blood of healthy children and pediatric patients with acute lymphoblastic leukemia. Our findings demonstrate for the first time that CD56(low)CD16(low) natural killer cells are more abundant in bone marrow than in peripheral blood and that their frequency is further increased in children with acute lymphoblastic leukemia. Bone marrow and peripheral blood CD56(low)CD16(low) natural killer cells compared with CD56(low)CD16(high) natural killer cells express lower levels of killer inhibitory receptors, higher levels of CD27, CD127, CD122, CD25, but undetectable levels of CD57, suggesting that they have a higher proliferative and differentiation potential. Moreover, CD56(low)CD16(low) natural killer cells display higher levels of CXCR4 and undetectable levels of CX3CR1 and can be consistently and rapidly mobilized in peripheral blood in response to CXCR4 antagonist. Unlike CD56(low)CD16(high), both bone marrow and peripheral blood CD56(low)CD16(low) natural killer cells release IFNγ following cytokine stimulation, and represent the major cytotoxic natural killer cell population against K562 or acute lymphoblastic leukemia target cells. All these data suggest that CD56(low)CD16(low) natural killer cells are multifunctional cells, and that the presence of hematologic malignancies affects their frequency and functional ability at both tumor site and in the periphery.<br /> (Copyright© Ferrata Storti Foundation.)
- Subjects :
- Adult
Age Factors
Bone Marrow Cells immunology
Case-Control Studies
Cell Degranulation immunology
Chemokines metabolism
Child
Child, Preschool
Cytotoxicity, Immunologic
Humans
Interferons biosynthesis
Killer Cells, Natural immunology
Leukemia immunology
Leukocytes, Mononuclear metabolism
Lymphocyte Count
Lymphocyte Subsets immunology
Phenotype
Platelet Glycoprotein GPIb-IX Complex metabolism
Bone Marrow Cells metabolism
CD56 Antigen metabolism
Immunophenotyping
Killer Cells, Natural metabolism
Leukemia metabolism
Lymphocyte Subsets metabolism
Receptors, IgG metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1592-8721
- Volume :
- 100
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Haematologica
- Publication Type :
- Academic Journal
- Accession number :
- 25596273
- Full Text :
- https://doi.org/10.3324/haematol.2014.116053