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Evaluation of a dansyl-based amino acid DNSBA as an imaging probe for apoptosis detection.
- Source :
-
Apoptosis : an international journal on programmed cell death [Apoptosis] 2015 Mar; Vol. 20 (3), pp. 410-20. - Publication Year :
- 2015
-
Abstract
- Imaging agents that enable direct detection of apoptosis are highly desirable in the field of monitoring chemotherapeutic response as well as early diagnosis and disease monitoring. Previous work demonstrated that the dansyled amino acid DNSBA is used to specifically and selectively detect apoptotic cancer cells at the both early and late stages, but the mechanism remains unclear. In this work, we evaluated DNSBA as a tool for monitoring cell apoptosis in CNE1 tumor cell models both in vitro and ex vivo after its in vivo administration, which was confirmed by other assays. The ability of DNSBA to detect multiple pathways and different stages of apoptosis leading to cell death may be advantageous in the evaluation of cancer treatment indicative of a positive therapeutic outcome. The uptake change of molecular probes DNSBA in CNE1 cells represented the changes of apoptotic rate in a caspase-dependent manner. However, the accumulation of DNSBA in apoptotic cells did not increase with the enhanced membrane permeability. Furthermore, ex vivo study demonstrated DNSBA has a similar pattern as the TUNEL-positive cells. In conclusion, DNSBA cellular imaging is useful for the early assessment of treatment-induced apoptosis, and thus may act as a substitute for Annexin V for assessing treatment response.
- Subjects :
- Animals
Annexin A5 genetics
Annexin A5 metabolism
Apoptosis drug effects
Biological Transport
Carcinoma
Caspases genetics
Caspases metabolism
Cell Line, Tumor
Cell Membrane Permeability
Dansyl Compounds chemistry
Gene Expression
Humans
In Situ Nick-End Labeling
Injections, Subcutaneous
Kinetics
Mice
Mice, Inbred BALB C
Molecular Probes chemistry
Nasopharyngeal Carcinoma
Nasopharyngeal Neoplasms diagnosis
Nasopharyngeal Neoplasms metabolism
Nasopharyngeal Neoplasms pathology
Neoplasm Transplantation
Paclitaxel pharmacology
Phenylpropionates chemistry
Tumor Necrosis Factor-alpha pharmacology
Dansyl Compounds metabolism
Molecular Probes metabolism
Nasopharyngeal Neoplasms genetics
Optical Imaging methods
Phenylpropionates metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-675X
- Volume :
- 20
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Apoptosis : an international journal on programmed cell death
- Publication Type :
- Academic Journal
- Accession number :
- 25596717
- Full Text :
- https://doi.org/10.1007/s10495-014-1075-z