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Regulation of a maize HD-ZIPĀ IV transcription factor by a non-conventional RDR2-dependent small RNA.

Authors :
Klein-Cosson C
Chambrier P
Rogowsky PM
Vernoud V
Source :
The Plant journal : for cell and molecular biology [Plant J] 2015 Mar; Vol. 81 (5), pp. 747-58.
Publication Year :
2015

Abstract

Small non-coding RNAs are versatile riboregulators that control gene expression at the transcriptional or post-transcriptional level, governing many facets of plant development. Here we present evidence for the existence of a 24 nt small RNA (named small1) that is complementary to the 3' UTR of OCL1 (Outer Cell Layer1), the founding member of the maize HD-ZIP IV gene family encoding plant-specific transcription factors that are mainly involved in epidermis differentiation and specialization. The biogenesis of small1 depends on DICER-like 3 (DCL3), RNA-dependent RNA polymerase 2 (RDR2) and RNA polymerase IV, components that are usually required for RNA-dependent DNA-methylation. Unexpectedly, GFP sensor experiments in transient and stable transformation systems revealed that small1 may regulate its target at the post-transcriptional level, mainly through translational repression. This translational repression is attenuated in an rdr2 mutant background in which small1 does not accumulate. Our experiments further showed the possible involvement of a secondary stem-loop structure present in the 3' UTR of OCL1 for efficient target repression, suggesting the existence of several regulatory mechanisms affecting OCL1 mRNA stability and translation.<br /> (© 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd.)

Details

Language :
English
ISSN :
1365-313X
Volume :
81
Issue :
5
Database :
MEDLINE
Journal :
The Plant journal : for cell and molecular biology
Publication Type :
Academic Journal
Accession number :
25619590
Full Text :
https://doi.org/10.1111/tpj.12771