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Regulation of a maize HD-ZIPĀ IV transcription factor by a non-conventional RDR2-dependent small RNA.
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 2015 Mar; Vol. 81 (5), pp. 747-58. - Publication Year :
- 2015
-
Abstract
- Small non-coding RNAs are versatile riboregulators that control gene expression at the transcriptional or post-transcriptional level, governing many facets of plant development. Here we present evidence for the existence of a 24 nt small RNA (named small1) that is complementary to the 3' UTR of OCL1 (Outer Cell Layer1), the founding member of the maize HD-ZIP IV gene family encoding plant-specific transcription factors that are mainly involved in epidermis differentiation and specialization. The biogenesis of small1 depends on DICER-like 3 (DCL3), RNA-dependent RNA polymerase 2 (RDR2) and RNA polymerase IV, components that are usually required for RNA-dependent DNA-methylation. Unexpectedly, GFP sensor experiments in transient and stable transformation systems revealed that small1 may regulate its target at the post-transcriptional level, mainly through translational repression. This translational repression is attenuated in an rdr2 mutant background in which small1 does not accumulate. Our experiments further showed the possible involvement of a secondary stem-loop structure present in the 3' UTR of OCL1 for efficient target repression, suggesting the existence of several regulatory mechanisms affecting OCL1 mRNA stability and translation.<br /> (© 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd.)
- Subjects :
- 3' Untranslated Regions genetics
DNA Methylation
Genes, Reporter
Protein Processing, Post-Translational
RNA, Messenger genetics
RNA, Plant genetics
RNA, Small Interfering
Gene Expression Regulation, Plant
Membrane Proteins genetics
Plant Proteins genetics
RNA-Dependent RNA Polymerase genetics
Transcription Factors genetics
Zea mays genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1365-313X
- Volume :
- 81
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 25619590
- Full Text :
- https://doi.org/10.1111/tpj.12771