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Assessment of short- and medium-chain fatty acids on mitochondrial function in severe inflammation.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1265, pp. 389-96. - Publication Year :
- 2015
-
Abstract
- Mitochondrial dysfunction is regarded as one key factor involved in the pathogenesis of septic disorders, leading to a decline in energy supply. The influence of short- and medium-chain fatty acids (SCFA/MCFA) on mitochondrial respiration under inflammatory conditions has thus far not been investigated. In the following protocol, we describe the assessment of mitochondrial respiration using high-resolution respirometry under inflammatory and baseline conditions. For this approach, human endothelial cells and monocytes were pretreated with TNF-α to mimic inflammation followed by incubation with SCFA/MCFA and then subjected to high-resolution respirometry. Mitochondrial DNA content was assessed by PCR.
- Subjects :
- Cells, Cultured
Fatty Acids pharmacology
Human Umbilical Vein Endothelial Cells drug effects
Human Umbilical Vein Endothelial Cells metabolism
Humans
Inflammation diagnosis
Monocytes drug effects
Monocytes metabolism
Oxygen Consumption
Severity of Illness Index
Fatty Acids metabolism
Inflammation metabolism
Mitochondria metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1265
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 25634290
- Full Text :
- https://doi.org/10.1007/978-1-4939-2288-8_28