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CsCTL1, a teleost C-type lectin that promotes antibacterial and antiviral immune defense in a manner that depends on the conserved EPN motif.
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2015 Jun; Vol. 50 (2), pp. 69-77. Date of Electronic Publication: 2015 Jan 28. - Publication Year :
- 2015
-
Abstract
- Many C-type lectins (CTLs) have been identified in teleost, however, the in vivo function of fish CTLs is essentially unknown. In this study, we examined the function of a CTL (CsCTL1) from tongue sole. CsCTL1 possesses the conserved EPN motif required for mannose binding in mammals but unknown in function in fish. Recombinant CsCTL1 (rCsCTL1), but not the mutant rCsCTL1M bearing substitutions at EPN, interacted with and agglutinated a limited range of bacteria. The agglutinating ability of rCsCTL1 was abolished in the absence of calcium or presence of mannose. Binding of rCsCTL1 to bacteria promoted phagocytosis and antimicrobial activity of head kidney monocytes. Fish administered with rCsCTL1 exhibited enhanced resistance against bacterial and viral infections. These results provide the first evidence that the EPN site is essential to a fish CTL and that, in addition to antibacterial properties, a fish CTL promotes the immune defense against viral infection as well.<br /> (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Subjects :
- Agglutination immunology
Amino Acid Motifs genetics
Amino Acid Sequence
Animals
Base Sequence
Edwardsiella tarda immunology
Fish Diseases immunology
Fish Diseases microbiology
Fish Diseases virology
Fish Proteins genetics
Fish Proteins immunology
Head Kidney cytology
Head Kidney immunology
Immunity, Innate genetics
Micrococcus luteus immunology
Monocytes immunology
Phagocytosis immunology
Recombinant Proteins pharmacology
Sequence Alignment
Sequence Analysis, DNA
Vibrio immunology
Anti-Bacterial Agents immunology
Antiviral Agents immunology
Flatfishes immunology
Lectins, C-Type genetics
Lectins, C-Type immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0089
- Volume :
- 50
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 25636784
- Full Text :
- https://doi.org/10.1016/j.dci.2015.01.007