Back to Search
Start Over
RARβ regulates neuronal cell death and differentiation in the avian ciliary ganglion.
- Source :
-
Developmental neurobiology [Dev Neurobiol] 2015 Nov; Vol. 75 (11), pp. 1204-18. Date of Electronic Publication: 2015 Jun 12. - Publication Year :
- 2015
-
Abstract
- Programmed cell death during chicken ciliary ganglion (CG) development is mostly discussed as an extrinsically regulated process, guided either by the establishment of a functional balance between preganglionic and postganglionic activity or the availability of target-derived neurotrophic factors. We found that the expression of the gene coding for the nuclear retinoic acid receptor β (RARB) is transiently upregulated prior to and during the execution phase of cell death in the CG. Using retroviral vectors, the expression of RARB was knocked down during embryonic development in ovo. The knockdown led to a significant increase in CG neuron number after the cell death phase. BrdU injections and active caspase-3 staining revealed that this increase in neuron number was due to an inhibition of apoptosis during the normal cell death phase. Furthermore, apoptotic neuron numbers were significantly increased at a stage when cell death is normally completed. While the cholinergic phenotype of the neurons remained unchanged after RARB knockdown, the expression of the proneural gene Cash1 was increased, but somatostatin-like immunoreactivity, a hallmark of the mature choroid neuron population, was decreased. Taken together, these results point toward a delay in neuronal differentiation as well as cell death. The availability of nuclear retinoic acid receptor β (RARβ) and RARβ-induced transcription of genes could therefore be a new intrinsic cue for the maturation of CG neurons and their predisposition to undergo cell death.<br /> (© 2015 The Authors Developmental Neurobiology Published by Wiley Periodicals, Inc.)
- Subjects :
- Animals
Basic Helix-Loop-Helix Transcription Factors metabolism
Caspase 3 metabolism
Cell Death physiology
Cells, Cultured
Chick Embryo
Choline O-Acetyltransferase metabolism
LIM-Homeodomain Proteins metabolism
Receptors, Retinoic Acid genetics
Transcription Factors metabolism
Avian Proteins metabolism
Ganglia, Parasympathetic embryology
Ganglia, Parasympathetic physiology
Neurogenesis physiology
Receptors, Retinoic Acid metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-846X
- Volume :
- 75
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Developmental neurobiology
- Publication Type :
- Academic Journal
- Accession number :
- 25663354
- Full Text :
- https://doi.org/10.1002/dneu.22278