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Overexpression of a Miscanthus lutarioriparius NAC gene MlNAC5 confers enhanced drought and cold tolerance in Arabidopsis.
- Source :
-
Plant cell reports [Plant Cell Rep] 2015 Jun; Vol. 34 (6), pp. 943-58. Date of Electronic Publication: 2015 Feb 10. - Publication Year :
- 2015
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Abstract
- Key Message: MLNAC5 functions as a stress-responsive NAC transcription factor gene and enhances drought and cold stress tolerance in transgenic Arabidopsis via the ABA-dependent signaling pathway. NAC transcription factors (TFs) play crucial roles in plant responses to abiotic stress. Miscanthus lutarioriparius is one of Miscanthus species native to East Asia. It has attracted much attention as a bioenergy crop because of its superior biomass productivity as well as wide adaptability to different environments. However, the functions of stress-related NAC TFs remain to be elucidated in M. lutarioriparius. In this study, a detailed functional characterization of MlNAC5 was carried out. MlNAC5 was a member of ATAF subfamily and it showed the highest sequence identity to ATAF1. Subcellular localization of MlNAC5-YFP fusion protein in tobacco leaves indicated that MlNAC5 is a nuclear protein. Transactivation assay in yeast cells demonstrated that MlNAC5 functions as a transcription activator and its activation domain is located in the C-terminus. Overexpression of MlNAC5 in Arabidopsis had impacts on plant development including dwarfism, leaf senescence, leaf morphology, and late flowering under normal growth conditions. Furthermore, MlNAC5 overexpression lines in Arabidopsis exhibited hypersensitivity to abscisic acid (ABA) and NaCl. Moreover, overexpression of MlNAC5 in Arabidopsis significantly enhanced drought and cold tolerance by transcriptionally regulating some stress-responsive marker genes. Collectively, our results indicated that MlNAC5 functions as an important regulator during the process of plant development and responses to salinity, drought and cold stresses.
- Subjects :
- Abscisic Acid metabolism
Arabidopsis genetics
Cell Nucleus metabolism
Cold-Shock Response genetics
Droughts
Gene Expression Regulation, Plant
Phylogeny
Plant Proteins genetics
Plant Proteins metabolism
Plants, Genetically Modified
Promoter Regions, Genetic
Salinity
Stress, Physiological genetics
Transcription Factors metabolism
Arabidopsis physiology
Poaceae genetics
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1432-203X
- Volume :
- 34
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Plant cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 25666276
- Full Text :
- https://doi.org/10.1007/s00299-015-1756-2