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ToF-SIMS observation for evaluating the interaction between amyloid β and lipid membranes.
- Source :
-
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2015 Apr; Vol. 407 (10), pp. 2859-63. Date of Electronic Publication: 2015 Feb 17. - Publication Year :
- 2015
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Abstract
- The adsorption behaviour of amyloid beta (Aβ), thought to be a key peptide for understanding Alzheimer's disease, was investigated by means of time-of-flight secondary ion mass spectrometry (ToF-SIMS). Aβ aggregates depending on the lipid membrane condition though it has not been fully understood yet. In this study, Aβ samples on different lipid membranes, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), were observed with ToF-SIMS and the complex ToF-SIMS data of the Aβ samples was interpreted using data analysis techniques such as principal component analysis (PCA), gentle-SIMS (G-SIMS) and g-ogram. DOPC and DMPC are liquid crystal at room temperature, while DPPC is gel at room temperature. As primary ion beams, Bi3(+) and Ar cluster ion beams were used and the effect of an Ar cluster ion for evaluating biomolecules was also studied. The secondary ion images of the peptide fragment ions indicated by G-SIMS and g-ogram were consistent with the PCA results. It is suggested that Aβ is adsorbed homogeneously on the liquid-crystalline-phase lipid membranes, while it aggregates along the lipid on the gel-phase lipid membrane. Moreover, in the results using the Ar cluster, the influence of contamination was reduced.
- Subjects :
- 1,2-Dipalmitoylphosphatidylcholine analogs & derivatives
1,2-Dipalmitoylphosphatidylcholine chemistry
Amino Acid Sequence
Amyloid beta-Peptides chemistry
Dimyristoylphosphatidylcholine chemistry
Glycerylphosphorylcholine analogs & derivatives
Glycerylphosphorylcholine chemistry
Image Processing, Computer-Assisted
Membrane Lipids metabolism
Molecular Sequence Data
Phosphatidylcholines
Principal Component Analysis
Amyloid beta-Peptides metabolism
Membrane Lipids chemistry
Spectrometry, Mass, Secondary Ion methods
Subjects
Details
- Language :
- English
- ISSN :
- 1618-2650
- Volume :
- 407
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Analytical and bioanalytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 25687682
- Full Text :
- https://doi.org/10.1007/s00216-015-8527-3