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[Actinobacteria and their odor-producing capacities in a surface water in Shanghai].
- Source :
-
Huan jing ke xue= Huanjing kexue [Huan Jing Ke Xue] 2014 Oct; Vol. 35 (10), pp. 3769-74. - Publication Year :
- 2014
-
Abstract
- The odor in raw water is one of the main sources of odor in drinking water. The occurrence of actinobacteria and their odor producing capacities in a reservoir in.Shanghai were investigated. Gauze's medium and membrane filtration were used for actinobacteria isolation. Through combined methods of 16S rRNA sequencing, colony and hyphae morphology, carbon source utilization, physiological and biochemical characteristics, 40 strains of actinobacteria were identified from the reservoir. Results showed that there were 38 Streptomyces, an Aeromicrobium and a Pseudonocardia. Liquid culture medium and the real reservoir water were used to test the odor producing capacity of these 40 strains of actinobacteria, and headspace solid phase microextraction (HS-SPME) and high resolution gas chromatography mass spectroscopy (GC/MS) were used to analyze the odor compounds 2-methylisoborneol (2-MIB) and geosmin (GSM) in the fermentation liquor. The test results showed that, the odor-producing capacities of these actinobacteria in different fermentation media showed different variation trends, even within the genera Streptomyces. The odor-producing capacity of actinobacteria in the liquid culture medium could not represent their states in the reservoir water or their actual odor contribution to the aquatic environment.
- Subjects :
- Actinobacteria isolation & purification
Camphanes analysis
China
Drinking Water microbiology
Gas Chromatography-Mass Spectrometry
Naphthols analysis
RNA, Ribosomal, 16S genetics
Solid Phase Microextraction
Actinobacteria classification
Drinking Water analysis
Odorants
Water Microbiology
Water Pollutants, Chemical analysis
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0250-3301
- Volume :
- 35
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Huan jing ke xue= Huanjing kexue
- Publication Type :
- Academic Journal
- Accession number :
- 25693381