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Macromolecular crowding-assisted fabrication of liquid-crystalline imprinted polymers.
- Source :
-
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2015 Apr; Vol. 407 (10), pp. 2923-31. Date of Electronic Publication: 2015 Feb 21. - Publication Year :
- 2015
-
Abstract
- A macromolecular crowding-assisted liquid-crystalline molecularly imprinted monolith (LC-MIM) was prepared successfully for the first time. The imprinted stationary phase was synthesized with polymethyl methacrylate (PMMA) or polystyrene (PS) as the crowding agent, 4-cyanophenyl dicyclohexyl propylene (CPCE) as the liquid-crystal monomer, and hydroquinidine as the pseudo-template for the chiral separation of cinchona alkaloids in HPLC. A low level of cross-linker (26%) has been found to be sufficient to achieve molecular recognition on the crowding-assisted LC-MIM due to the physical cross-linking of mesogenic groups in place of chemical cross-linking, and baseline separation of quinidine and quinine could be achieved with good resolution (R(s) = 2.96), selectivity factor (α = 2.16), and column efficiency (N = 2650 plates/m). In contrast, the LC-MIM prepared without crowding agents displayed the smallest diastereoselectivity (α = 1.90), while the crowding-assisted MIM with high level of cross-linker (80%) obtained the greatest selectivity factor (α = 7.65), but the lowest column efficiency (N = 177 plates/m).
- Subjects :
- Acetonitriles chemistry
Chemistry Techniques, Synthetic
Cinchona Alkaloids analysis
Cinchona Alkaloids isolation & purification
Cross-Linking Reagents chemistry
Hydrogen-Ion Concentration
Polymethyl Methacrylate chemistry
Quinidine analogs & derivatives
Quinidine analysis
Quinidine chemistry
Quinine analysis
Temperature
Chromatography, High Pressure Liquid methods
Liquid Crystals chemistry
Molecular Imprinting methods
Polystyrenes chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1618-2650
- Volume :
- 407
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Analytical and bioanalytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 25701416
- Full Text :
- https://doi.org/10.1007/s00216-015-8510-z