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Structural Requirements of Strigolactones for Shoot Branching Inhibition in Rice and Arabidopsis.
Structural Requirements of Strigolactones for Shoot Branching Inhibition in Rice and Arabidopsis.
- Source :
-
Plant & cell physiology [Plant Cell Physiol] 2015 Jun; Vol. 56 (6), pp. 1059-72. Date of Electronic Publication: 2015 Feb 23. - Publication Year :
- 2015
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Abstract
- The structural requirements of strigolactones (SLs) involved in germination induction of root parasitic plants and hyphal branching in arbuscular mycorrhizal (AM) fungi have been extensively studied. However, our knowledge of the requirements of SLs involved in shoot branching inhibition in plants is still limited. To address this question, we investigated the structure-activity relationships of SLs in shoot branching inhibition in rice and Arabidopsis. SLs possess a four-ring structure, with a tricyclic lactone (ABC-rings) connected to a methylbutenolide part (D-ring) via an enol ether bridge. Here, we show that the the (R) configuration at C-2', which determines the steric position of the D-ring relative to the enol ether olefin bond, is critical for the hormonal activity in rice. Replacement of the enol ether moiety by an alkoxy or imino ether resulted in a severe reduction in biological activity in rice. Moreover, yeast two-hybrid experiments using a possible SL receptor, DWARF14 (D14), and a repressor in the SL signaling pathway, DWARF53 (D53), showed that D14 can interact with D53 in the presence of (2'R) stereoisomers of SLs, but not (2'S) stereoisomers, suggesting that the stereostructure of SLs is crucial for the interaction of these proteins. When GR5, an AB-ring-truncated analog, was applied to the hydroponic culture medium, strong inhibition of shoot branching was observed both in rice and in Arabidopsis. However, GR5 was only weakly active when directly applied to the axillary buds of Arabidopsis. Our results indicate that the difference in plant species and application methods greatly influences the apparent SL biological activity.<br /> (© The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
- Subjects :
- Arabidopsis drug effects
Culture Media
Ether chemistry
Germination drug effects
Hydroponics
Hypocotyl drug effects
Hypocotyl growth & development
Lactones chemistry
Oryza drug effects
Plant Shoots drug effects
Seeds drug effects
Seeds growth & development
Stereoisomerism
Two-Hybrid System Techniques
Arabidopsis growth & development
Lactones pharmacology
Oryza growth & development
Plant Shoots growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1471-9053
- Volume :
- 56
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Plant & cell physiology
- Publication Type :
- Academic Journal
- Accession number :
- 25713176
- Full Text :
- https://doi.org/10.1093/pcp/pcv028