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New monoclonal antibodies specific for 1-(5-fluoropentyl)-3-(2-iodobenzoyl)indole.
- Source :
-
Monoclonal antibodies in immunodiagnosis and immunotherapy [Monoclon Antib Immunodiagn Immunother] 2015 Feb; Vol. 34 (1), pp. 55-8. - Publication Year :
- 2015
-
Abstract
- 1-(5-fluoropentyl)-3-(2-iodobenzoyl)indole (AM694) is one of the synthetic cannabinoids and an illegal drug in Japan. It is important to generate a monoclonal antibody (MAb) against AM694 for use in the rapid and sensitive detection of the drug. Two monoclonal antibodies, named HN0124 (IgG1) and NK0504 (IgG1), were obtained, which were possibly effective for detecting AM694 and its derivatives. The cross-reactive ability of these MAbs was evaluated using a competitive enzyme-linked immunosorbent assay. In the results, both of these antibodies recognize 1-(5-fluoropentyl)-3-(2-iodobenzoyl)indole, 1-(5-fluoropentyl)-3-(3-iodobenzoyl)indole, 1-(5-fluoropentyl)-3-(4-iodobenzoyl)indole. Forty nmol/L AM694 can be detected using HN0124 MAb. Thus, MAbs produced in this study could be considered a useful tool for the detection of AM694.
- Subjects :
- Animals
Antibodies, Monoclonal biosynthesis
Antibody Specificity
Binding, Competitive
Cross Reactions
Enzyme-Linked Immunosorbent Assay methods
Female
Haptens chemistry
Haptens immunology
Hemocyanins chemistry
Hemocyanins immunology
Humans
Hybridomas immunology
Immunization
Immunoconjugates administration & dosage
Immunoconjugates chemistry
Immunoconjugates immunology
Immunoglobulin G biosynthesis
Japan
Limit of Detection
Mice
Antibodies, Monoclonal chemistry
Cannabinoid Receptor Agonists analysis
Illicit Drugs analysis
Immunoglobulin G chemistry
Indoles analysis
Subjects
Details
- Language :
- English
- ISSN :
- 2167-9436
- Volume :
- 34
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Monoclonal antibodies in immunodiagnosis and immunotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 25723285
- Full Text :
- https://doi.org/10.1089/mab.2014.0076