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Polyphosphoinositide binding domains: Key to inositol lipid biology.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 2015 Jun; Vol. 1851 (6), pp. 746-58. Date of Electronic Publication: 2015 Feb 27. - Publication Year :
- 2015
-
Abstract
- Polyphosphoinositides (PPIn) are an important family of phospholipids located on the cytoplasmic leaflet of eukaryotic cell membranes. Collectively, they are critical for the regulation of many aspects of membrane homeostasis and signaling, with notable relevance to human physiology and disease. This regulation is achieved through the selective interaction of these lipids with hundreds of cellular proteins, and thus the capability to study these localized interactions is crucial to understanding their functions. In this review, we discuss current knowledge of the principle types of PPIn-protein interactions, focusing on specific lipid-binding domains. We then discuss how these domains have been re-tasked by biologists as molecular probes for these lipids in living cells. Finally, we describe how the knowledge gained with these probes, when combined with other techniques, has led to the current view of the lipids' localization and function in eukaryotes, focusing mainly on animal cells. This article is part of a Special Issue entitled Phosphoinositides.<br /> (Published by Elsevier B.V.)
- Subjects :
- Adaptor Proteins, Signal Transducing chemistry
Adaptor Proteins, Signal Transducing genetics
Adaptor Proteins, Signal Transducing metabolism
Animals
Cell Membrane chemistry
Cell Membrane metabolism
Green Fluorescent Proteins chemistry
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
Guanine Nucleotide Exchange Factors chemistry
Guanine Nucleotide Exchange Factors genetics
Guanine Nucleotide Exchange Factors metabolism
Humans
Molecular Imaging
Molecular Probes metabolism
Phosphatidylinositols metabolism
Protein Binding
Protein Structure, Tertiary
Recombinant Fusion Proteins chemistry
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Signal Transduction
Tacrolimus Binding Proteins chemistry
Tacrolimus Binding Proteins genetics
Tacrolimus Binding Proteins metabolism
Type C Phospholipases chemistry
Type C Phospholipases genetics
Type C Phospholipases metabolism
Biosensing Techniques
Molecular Probes chemistry
Phosphatidylinositols analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 1851
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 25732852
- Full Text :
- https://doi.org/10.1016/j.bbalip.2015.02.013