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Three R2R3-MYB transcription factors regulate distinct floral pigmentation patterning in Phalaenopsis spp.
- Source :
-
Plant physiology [Plant Physiol] 2015 May; Vol. 168 (1), pp. 175-91. Date of Electronic Publication: 2015 Mar 04. - Publication Year :
- 2015
-
Abstract
- Orchidaceae are well known for their fascinating floral morphologic features, specialized pollination, and distinctive ecological strategies. With their long-lasting flowers of various colors and pigmentation patterning, Phalaenopsis spp. have become important ornamental plants worldwide. In this study, we identified three R2R3-MYB transcription factors PeMYB2, PeMYB11, and PeMYB12. Their expression profiles were concomitant with red color formation in Phalaenopsis spp. flowers. Transient assay of overexpression of three PeMYBs verified that PeMYB2 resulted in anthocyanin accumulation, and these PeMYBs could activate the expression of three downstream structural genes Phalaenopsis spp. Flavanone 3-hydroxylase5, Phalaenopsis spp. Dihydroflavonol 4-reductase1, and Phalaenopsis spp. Anthocyanidin synthase3. In addition, these three PeMYBs participated in the distinct pigmentation patterning in a single flower, which was revealed by virus-induced gene silencing. In the sepals/petals, silencing of PeMYB2, PeMYB11, and PeMYB12 resulted in the loss of the full-red pigmentation, red spots, and venation patterns, respectively. Moreover, different pigmentation patterning was regulated by PeMYBs in the sepals/petals and lip. PeMYB11 was responsive to the red spots in the callus of the lip, and PeMYB12 participated in the full pigmentation in the central lobe of the lip. The differential pigmentation patterning was validated by RNA in situ hybridization. Additional assessment was performed in six Phalaenopsis spp. cultivars with different color patterns. The combined expression of these three PeMYBs in different ratios leads to a wealth of complicated floral pigmentation patterning in Phalaenopsis spp.<br /> (© 2015 American Society of Plant Biologists. All Rights Reserved.)
- Subjects :
- Amino Acid Sequence
Anthocyanins biosynthesis
Flowers genetics
Flowers metabolism
Gene Expression Profiling
Gene Expression Regulation, Plant
Genes, Plant
In Situ Hybridization
Models, Biological
Molecular Sequence Data
Orchidaceae genetics
Phenotype
Phylogeny
Plant Proteins chemistry
Plant Proteins genetics
Plant Proteins isolation & purification
RNA, Plant genetics
Real-Time Polymerase Chain Reaction
Reproducibility of Results
Sequence Alignment
Transcription Factors chemistry
Transcription Factors genetics
Transcription Factors isolation & purification
Body Patterning
Flowers growth & development
Orchidaceae growth & development
Orchidaceae metabolism
Pigmentation genetics
Plant Proteins metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2548
- Volume :
- 168
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 25739699
- Full Text :
- https://doi.org/10.1104/pp.114.254599