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A FRET based pH probe with a broad working range applicable to referenced ratiometric dual wavelength and luminescence lifetime read out.

Authors :
Meier RJ
Simbürger JM
Soukka T
Schäferling M
Source :
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2015 Apr 11; Vol. 51 (28), pp. 6145-8.
Publication Year :
2015

Abstract

A luminescent probe for determination of pH was designed based on a Förster resonance energy transfer (FRET) system, combining a europium chelate as the donor and carboxynaphtho-fluorescein as a pH sensitive acceptor. The FRET system enables referenced pH detection in an exceptional broad dynamic range from pH 3 to 9.

Details

Language :
English
ISSN :
1364-548X
Volume :
51
Issue :
28
Database :
MEDLINE
Journal :
Chemical communications (Cambridge, England)
Publication Type :
Academic Journal
Accession number :
25747771
Full Text :
https://doi.org/10.1039/c5cc00144g