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Comparative Proteomics of Human and Macaque Milk Reveals Species-Specific Nutrition during Postnatal Development.
- Source :
-
Journal of proteome research [J Proteome Res] 2015 May 01; Vol. 14 (5), pp. 2143-57. Date of Electronic Publication: 2015 Apr 06. - Publication Year :
- 2015
-
Abstract
- Milk has been well established as the optimal nutrition source for infants, yet there is still much to be understood about its molecular composition. Therefore, our objective was to develop and compare comprehensive milk proteomes for human and rhesus macaques to highlight differences in neonatal nutrition. We developed a milk proteomics technique that overcomes previous technical barriers including pervasive post-translational modifications and limited sample volume. We identified 1606 and 518 proteins in human and macaque milk, respectively. During analysis of detected protein orthologs, we identified 88 differentially abundant proteins. Of these, 93% exhibited increased abundance in human milk relative to macaque and include lactoferrin, polymeric immunoglobulin receptor, alpha-1 antichymotrypsin, vitamin D-binding protein, and haptocorrin. Furthermore, proteins more abundant in human milk compared with macaque are associated with development of the gastrointestinal tract, the immune system, and the brain. Overall, our novel proteomics method reveals the first comprehensive macaque milk proteome and 524 newly identified human milk proteins. The differentially abundant proteins observed are consistent with the perspective that human infants, compared with nonhuman primates, are born at a slightly earlier stage of somatic development and require additional support through higher quantities of specific proteins to nurture human infant maturation.
- Subjects :
- Animals
Brain growth & development
Brain metabolism
Child Development physiology
Chromatography, Liquid
Female
Gastrointestinal Tract growth & development
Gastrointestinal Tract metabolism
Humans
Immune System growth & development
Immune System metabolism
Infant
Lactoferrin isolation & purification
Lactoferrin metabolism
Macaca mulatta growth & development
Macaca mulatta metabolism
Milk, Human metabolism
Proteome metabolism
Receptors, Polymeric Immunoglobulin isolation & purification
Receptors, Polymeric Immunoglobulin metabolism
Species Specificity
Tandem Mass Spectrometry
Transcobalamins isolation & purification
Transcobalamins metabolism
Vitamin D-Binding Protein isolation & purification
Vitamin D-Binding Protein metabolism
alpha 1-Antichymotrypsin isolation & purification
alpha 1-Antichymotrypsin metabolism
Lactation physiology
Milk, Human chemistry
Molecular Sequence Annotation
Proteome isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1535-3907
- Volume :
- 14
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of proteome research
- Publication Type :
- Academic Journal
- Accession number :
- 25757574
- Full Text :
- https://doi.org/10.1021/pr501243m