A highly sensitive and class-specific fluorescence polarisation assay for sulphonamides based on dihydropteroate synthase.

Authors :: Wang Z
Liang X
Wen K
Zhang S
Li C
Shen J
Source :: Biosensors & bioelectronics [Biosens Bioelectron] 2015 Aug 15; Vol. 70, pp. 1-4. Date of Electronic Publication: 2015 Mar 10.
Publication Year :: 2015
Abstract: We describe a fluorescence polarisation assay based on the use of dihydropteroate synthase (DHPS) and a fluorescence probe for multi-sulphonamide detection. Dihydropteridine pyrophosphate (DHPPP) was synthesised and acts as the first substrate for DHPS. Under optimised conditions, the half-maximal inhibitory concentrations (IC50) of the assay were less than 100 ng mL(-1) for at least 29 sulphonamides and the time needed for the detection was less than 20 min. More importantly, the assay revealed quite uniform affinities for all of the individual sulphonamides tested, which has never before been achieved in an antibody-based assay.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)

Subjects :: Animals
Anti-Bacterial Agents analysis
Anti-Bacterial Agents immunology
Dihydropteroate Synthase immunology
Equipment Design
Equipment Failure Analysis
Fluorescent Dyes chemistry
Food Contamination analysis
Reproducibility of Results
Sensitivity and Specificity
Sulfonamides immunology
Dihydropteroate Synthase chemistry
Fluorescence Polarization Immunoassay instrumentation
Food Analysis instrumentation
Milk chemistry
Phosphoric Acids chemistry
Pterins chemistry
Sulfonamides analysis

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