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Effects of TiO₂ and Co₃O₄ nanoparticles on circulating angiogenic cells.
Effects of TiO₂ and Co₃O₄ nanoparticles on circulating angiogenic cells.
- Source :
-
PloS one [PLoS One] 2015 Mar 24; Vol. 10 (3), pp. e0119310. Date of Electronic Publication: 2015 Mar 24 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- Background and Aim: Sparse evidence suggests a possible link between exposure to airborne nanoparticles (NPs) and cardiovascular (CV) risk, perhaps through mechanisms involving oxidative stress and inflammation. We assessed the effects of TiO2 and Co3O4 NPs in human circulating angiogenic cells (CACs), which take part in vascular endothelium repair/replacement.<br />Methods: CACs were isolated from healthy donors' buffy coats after culturing lymphomonocytes on fibronectin-coated dishes in endothelial medium for 7 days. CACs were pre-incubated with increasing concentration of TiO2 and Co3O4 (from 1 to 100 μg/ml) to test the effects of NP – characterized by Transmission Electron Microscopy – on CAC viability, apoptosis (caspase 3/7 activation), function (fibronectin adhesion assay), oxidative stress and inflammatory cytokine gene expression.<br />Results: Neither oxidative stress nor cell death were associated with exposure to TiO2 NP (except at the highest concentration tested), which, however, induced a higher pro-inflammatory effect compared to Co3O4 NPs (p<0.01). Exposure to Co3O4 NPs significantly reduced cell viability (p<0.01) and increased caspase activity (p<0.01), lipid peroxidation end-products (p<0.05) and pro-inflammatory cytokine gene expression (p<0.05 or lower). Notably, CAC functional activity was impaired after exposure to both TiO2 (p<0.05 or lower) and Co3O4 (p<0.01) NPs.<br />Conclusions: In vitro exposure to TiO2 and Co3O4 NPs exerts detrimental effects on CAC viability and function, possibly mediated by accelerated apoptosis, increased oxidant stress (Co3O4 NPs only) and enhancement of inflammatory pathways (both TiO2 and Co3O4 NPs). Such adverse effects may be relevant for a potential role of exposure to TiO2 and Co3O4 NPs in enhancing CV risk in humans.
- Subjects :
- Cobalt chemistry
Cobalt toxicity
Endothelium, Vascular cytology
Endothelium, Vascular drug effects
Humans
Leukocytes, Mononuclear cytology
Metal Nanoparticles administration & dosage
Metal Nanoparticles chemistry
Metal Nanoparticles toxicity
Microscopy, Electron, Transmission
Oxides chemistry
Oxides toxicity
Primary Cell Culture
Titanium chemistry
Titanium toxicity
Apoptosis drug effects
Cobalt pharmacology
Leukocytes, Mononuclear drug effects
Neovascularization, Physiologic drug effects
Oxidative Stress drug effects
Oxides pharmacology
Titanium pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 25803285
- Full Text :
- https://doi.org/10.1371/journal.pone.0119310