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Inhibitory activities of caffeoylquinic acid derivatives from Ilex kudingcha C.J. Tseng on α-glucosidase from Saccharomyces cerevisiae.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2015 Apr 15; Vol. 63 (14), pp. 3694-703. Date of Electronic Publication: 2015 Apr 07. - Publication Year :
- 2015
-
Abstract
- Polyphenols and caffeoylquinic acid (CQA) derivatives (3-CQA, 4-CQA, 5-CQA, 3,4-diCQA, 3,5-diCQA, and 4,5-diCQA) were prepared from Ilex kudingcha C.J. Tseng, and their effects and mechanisms on the activities of α-glucosidase from Saccharomyces cerevisiae were investigated in the present study. As results, the IC50 values for CQA derivatives were 0.16-0.39 mg/mL, and the inhibition mode of CQA derivatives was noncompetitive. On the basis of fluorescence spectroscopy and circular dichroism spectroscopy data, the binding constants and number of binding sites were calculated to be 10(6)-10(8) M(-1) and 1.42-1.87, respectively. CQA derivatives could bind to the enzyme mainly through hydrophobic interaction, altering the microenvironment and molecular conformation of the enzyme, thus decreasing the catalytic activity. To the authors' knowledge, this is the first report on α-glucosidase inhibitory mechanism by CQA derivatives from I. kudingcha, and the findings suggest a potential use of kudingcha as functional foods for the prevention and treatment of diabetes and related symptoms.
- Subjects :
- Glycoside Hydrolase Inhibitors isolation & purification
Kinetics
Molecular Structure
Plant Extracts isolation & purification
Quinic Acid chemistry
Quinic Acid isolation & purification
Saccharomyces cerevisiae Proteins chemistry
alpha-Glucosidases chemistry
Glycoside Hydrolase Inhibitors chemistry
Ilex chemistry
Plant Extracts chemistry
Quinic Acid analogs & derivatives
Saccharomyces cerevisiae enzymology
Saccharomyces cerevisiae Proteins antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 63
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 25805337
- Full Text :
- https://doi.org/10.1021/acs.jafc.5b00420