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The F-box protein Fbp1 functions in the invasive growth and cell wall integrity mitogen-activated protein kinase (MAPK) pathways in Fusarium oxysporum.
- Source :
-
Molecular plant pathology [Mol Plant Pathol] 2016 Jan; Vol. 17 (1), pp. 55-64. Date of Electronic Publication: 2015 Apr 28. - Publication Year :
- 2016
-
Abstract
- F-box proteins determine substrate specificity of the ubiquitin-proteasome system. Previous work has demonstrated that the F-box protein Fbp1, a component of the SCF(Fbp1) E3 ligase complex, is essential for invasive growth and virulence of the fungal plant pathogen Fusarium oxysporum. Here, we show that, in addition to invasive growth, Fbp1 also contributes to vegetative hyphal fusion and fungal adhesion to tomato roots. All of these functions have been shown previously to require the mitogen-activated protein kinase (MAPK) Fmk1. We found that Fbp1 is required for full phosphorylation of Fmk1, indicating that Fbp1 regulates virulence and invasive growth via the Fmk1 pathway. Moreover, the Δfbp1 mutant is hypersensitive to sodium dodecylsulfate (SDS) and calcofluor white (CFW) and shows reduced phosphorylation levels of the cell wall integrity MAPK Mpk1 after SDS treatment. Collectively, these results suggest that Fbp1 contributes to both the invasive growth and cell wall integrity MAPK pathways of F. oxysporum.<br /> (© 2015 BSPP AND JOHN WILEY & SONS LTD.)
- Subjects :
- Fusarium cytology
Fusarium genetics
Gene Expression Regulation, Fungal
Hydrophobic and Hydrophilic Interactions
Hyphae physiology
Mutation
Pectins metabolism
Plant Roots microbiology
Stress, Physiological
Cell Wall enzymology
Fungal Proteins metabolism
Fusarium growth & development
Fusarium pathogenicity
MAP Kinase Signaling System
Mitogen-Activated Protein Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1364-3703
- Volume :
- 17
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular plant pathology
- Publication Type :
- Academic Journal
- Accession number :
- 25808603
- Full Text :
- https://doi.org/10.1111/mpp.12259