Back to Search
Start Over
Serotype shift of a 793/B genotype infectious bronchitis coronavirus by natural recombination.
- Source :
-
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2015 Jun; Vol. 32, pp. 377-87. Date of Electronic Publication: 2015 Apr 03. - Publication Year :
- 2015
-
Abstract
- An infectious bronchitis coronavirus, designated as ck/CH/LHLJ/140906, was isolated from an infectious bronchitis virus (IBV) strain H120-vaccinated chicken flock, which presented with a suspected infectious bronchitis virus (IBV) infection. A phylogenetic analysis based on the S1 gene clustered ck/CH/LHLJ/140906 with the 793/B group; however, a pairwise comparison showed that the 5' terminal of the S1 gene (containing hypervariable regions I and II) had high sequence identity with the H120 strain, while the 3' terminal sequence was very similar to that of IBV 4/91 strain. A SimPlot analysis of the complete genomic sequence, which was confirmed by a phylogenetic analysis and nucleotide similarities using the corresponding gene fragments, suggested that isolate ck/CH/LHLJ/140906 emerged from multiple recombination events between parental IBV strains 4/91 and H120. Although the isolate ck/CH/LHLJ/140906 had slightly higher S1 amino acid sequence identity to strain 4/91 (88.2%) than to strain H120 (86%), the serotype of the virus was more closely related to that of the H120 strain (32% antigenic relatedness) than to the 4/91 strain (15% antigenic relatedness). Whereas, vaccination of specific pathogen-free chickens with the 4/91 vaccine provided better protection against challenge with ck/CH/LHLJ/140906 than did vaccination with the H120 strain according to the result of virus re-isolation. As the spike protein, especially in the hypervariable regions of the S1 domain, of IBVs contains viral neutralizing epitopes, the results of this study showed that recombination of the S1 domain resulted in the emergence of a new serotype.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Bronchitis veterinary
Coronavirus classification
Coronavirus isolation & purification
Genes, Viral
Genomics
Molecular Sequence Data
Multigene Family
Phylogeny
Poultry Diseases virology
RNA, Viral genetics
Sequence Analysis, RNA
Serogroup
Vaccination
Bronchitis virology
Chickens virology
Coronavirus genetics
Coronavirus Infections veterinary
Genotype
Recombination, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1567-7257
- Volume :
- 32
- Database :
- MEDLINE
- Journal :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 25843651
- Full Text :
- https://doi.org/10.1016/j.meegid.2015.03.034