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Xuezhikang () reduced renal cell apoptosis in streptozocin-induced diabetic rats through regulation of Bcl-2 family.
- Source :
-
Chinese journal of integrative medicine [Chin J Integr Med] 2016 Aug; Vol. 22 (8), pp. 611-8. Date of Electronic Publication: 2015 Apr 06. - Publication Year :
- 2016
-
Abstract
- Objective: To investigate the effect of Xuezhikang (, XZK) on renal cell apoptosis in diabetic rats and the possible mechanism.<br />Methods: Sixty-six rats were randomly divided into 3 groups: the normal, model and XZK groups. In each group, the rats were further randomly divided into 3-month and 6-month subgroups, respectively. Diabetes of rats was induced by a single intraperitoneal injection of 1% streptozocin at 60 mg/kg body weight. Rats in the XZK group received gastric perfusion of XZK (1200 mg/kg body weight) everyday for 3 or 6 months, while rats in the normal and model groups received equal volume of saline. Twenty-four hours' urine was collected for urinary albumin excretion (UAE) measurement. Periodic acid-Schiff (PAS) and Masson's trichrome staining were used for saccharides and collagen detection. Cell apoptosis of renal cortex was investigated by TdT-mediated dUTP nick end labeling (TUNEL) staining. Bax and Bcl-2 expressions were detected by immunohistochemistry and Western blot, respectively. Cytochrome C (Cyt C) and caspase-9 concentration were detected by Western blot.<br />Results: Compared with the model group, XZK treatment could significantly decrease the kidney hypertrophy index, 24 h UAE, renal cell apoptosis, cytoplasmic Cyt C level and active caspase-9 level, as well as suppress the increment of Bax and up-regulate the expression of Bcl-2, leading to the suppression of Bax/Bcl-2 ratio at 3 and 6 months (P<0.05 or P<0.01). Moreover, XZK treatment could alleviate the deposition of PAS-stained saccharides and Masson's trichromestained collagen to different extent.<br />Conclusions: Renal cell apoptosis was observed in diabetic kidney, in which mitochondrial apoptotic pathway might be involved. XZK treatment could attenuate pathological changes in diabetic kidney and reduce renal cell apoptosis, probably via the suppression of Bax/Bcl-2 ratio, which lead to inhibition of Cyt C release and following caspase-9 activation.
- Subjects :
- Albuminuria blood
Albuminuria complications
Animals
Blood Glucose metabolism
Caspase 9 metabolism
Cytochromes c metabolism
Diabetes Mellitus, Experimental blood
Diabetes Mellitus, Experimental metabolism
Drugs, Chinese Herbal pharmacology
Hypertrophy
In Situ Nick-End Labeling
Kidney drug effects
Kidney Glomerulus pathology
Lipids blood
Male
Mesangial Cells drug effects
Mesangial Cells pathology
Rats, Sprague-Dawley
Streptozocin
bcl-2-Associated X Protein metabolism
Apoptosis drug effects
Diabetes Mellitus, Experimental drug therapy
Diabetes Mellitus, Experimental pathology
Drugs, Chinese Herbal therapeutic use
Kidney pathology
Proto-Oncogene Proteins c-bcl-2 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1993-0402
- Volume :
- 22
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Chinese journal of integrative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 25847779
- Full Text :
- https://doi.org/10.1007/s11655-015-2050-4