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Essential roles of Cdc42 and MAPK in cadmium-induced apoptosis in Litopenaeus vannamei.
- Source :
-
Aquatic toxicology (Amsterdam, Netherlands) [Aquat Toxicol] 2015 Jun; Vol. 163, pp. 89-96. Date of Electronic Publication: 2015 Apr 01. - Publication Year :
- 2015
-
Abstract
- Cadmium, one of the most toxic heavy metals in aquatic environments, has severe effects on marine invertebrates and fishes. The MAPK signaling pathway plays a vital role in stress responses of animals. The mitogen-activated protein kinase (MAPK) signaling pathway plays a vital role in animals' stress responses, including mediation of apoptosis induced by the Rho GTPase Cdc42. However, there is limited knowledge about its function in shrimps, although disorders exacerbated by environmental stresses (including heavy metal pollution) have caused serious mortality in commercially cultured shrimps. Thus, we probed roles of Cdc42 in Litopenaeus vannamei shrimps (LvCdc42) during cadmium exposure by inhibiting its expression using dsRNA-mediated RNA interference. The treatment successfully reduced expression levels of MAPKs (including p38, JNK, and ERK). Cadmium exposure induced significant increases in expression levels of LvCdc42 and MAPKs, accompanied by reductions in total hemocyte counts (THC) and increases in apoptotic hemocyte ratios and ROS production. However, all of these responses were much weaker in LvCdc42-suppressed shrimps, in which mortality rates were higher than in controls. Our results suggest that the MAPK pathway plays a vital role in shrimps' responses to Cd(2+). They also indicate that LvCdc42 in shrimps participates in its regulation, and thus plays key roles in ROS production, regulation of apoptosis and associated stress responses.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Decapoda metabolism
Hemocytes cytology
Hemocytes drug effects
Hemocytes metabolism
RNA Interference
RNA, Double-Stranded metabolism
Reactive Oxygen Species metabolism
Signal Transduction drug effects
cdc42 GTP-Binding Protein antagonists & inhibitors
cdc42 GTP-Binding Protein genetics
Apoptosis drug effects
Cadmium toxicity
Decapoda drug effects
Mitogen-Activated Protein Kinases metabolism
Water Pollutants, Chemical toxicity
cdc42 GTP-Binding Protein metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1514
- Volume :
- 163
- Database :
- MEDLINE
- Journal :
- Aquatic toxicology (Amsterdam, Netherlands)
- Publication Type :
- Academic Journal
- Accession number :
- 25863597
- Full Text :
- https://doi.org/10.1016/j.aquatox.2015.03.023