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Tools for Anopheles gambiae Transgenesis.
- Source :
-
G3 (Bethesda, Md.) [G3 (Bethesda)] 2015 Apr 13; Vol. 5 (6), pp. 1151-63. Date of Electronic Publication: 2015 Apr 13. - Publication Year :
- 2015
-
Abstract
- Transgenesis is an essential tool to investigate gene function and to introduce desired characters in laboratory organisms. Setting-up transgenesis in non-model organisms is challenging due to the diversity of biological life traits and due to knowledge gaps in genomic information. Some procedures will be broadly applicable to many organisms, and others have to be specifically developed for the target species. Transgenesis in disease vector mosquitoes has existed since the 2000s but has remained limited by the delicate biology of these insects. Here, we report a compilation of the transgenesis tools that we have designed for the malaria vector Anopheles gambiae, including new docking strains, convenient transgenesis plasmids, a puromycin resistance selection marker, mosquitoes expressing cre recombinase, and various reporter lines defining the activity of cloned promoters. This toolbox contributed to rendering transgenesis routine in this species and is now enabling the development of increasingly refined genetic manipulations such as targeted mutagenesis. Some of the reagents and procedures reported here are easily transferable to other nonmodel species, including other disease vector or agricultural pest insects.<br /> (Copyright © 2015 Volohonsky et al.)
- Subjects :
- Animals
Animals, Genetically Modified
Cloning, Molecular
Codon genetics
DNA Transposable Elements genetics
Embryo, Nonmammalian metabolism
Flow Cytometry
Genes, Insect
Genes, Reporter
Genetic Vectors metabolism
Integrases metabolism
Larva genetics
Microinjections
Mosaicism
Ovum metabolism
Plasmids genetics
Promoter Regions, Genetic genetics
Transgenes
Transposases metabolism
Anopheles genetics
Gene Transfer Techniques
Subjects
Details
- Language :
- English
- ISSN :
- 2160-1836
- Volume :
- 5
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- G3 (Bethesda, Md.)
- Publication Type :
- Academic Journal
- Accession number :
- 25869647
- Full Text :
- https://doi.org/10.1534/g3.115.016808