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Establishment and characterization of GCSR1, a multi-drug resistant signet ring cell gastric cancer cell line.
- Source :
-
International journal of oncology [Int J Oncol] 2015; Vol. 46 (6), pp. 2479-87. Date of Electronic Publication: 2015 Apr 16. - Publication Year :
- 2015
-
Abstract
- Signet ring cell gastric cancer (SRCGC) has very poor prognosis worldwide, and studying its molecular characteristics is urgent for improving the outcome. However, few well-characterized SRCGC cell lines are available for research. Therefore, we established a novel cell line GCSR1, from a Chinese male SRCGC patient. Cell morphology of GCSR1 in culture, maintained in vitro for over 90 passages, is similar to the cells from the patient. GCSR1 cells proliferated in vitro with a doubling time of 67.65 h. Karyotyping showed they were aneuploid. Missense mutation occurred in codon 193 of P53 and deletion occurred in exons 1 and 3 of P16. Results of CCK8 assay revealed that GCSR1 was more resistant to 5-fluorouracil (5-FU) and mitomycin (MMC) than other gastric cancer cell lines. Stem cell marker assay by flow cytometry showed that GCSR1 had high proportion of CD44+ and/or CD133+ cells. It formed colonies easily in soft agar and generated xenograft tumors in nude mice. In conclusion, GCSR1 is a well-established, well-characterized multi-drug resistant cell line with abundant cancer stem cells.
- Subjects :
- Animals
Carcinoma, Signet Ring Cell genetics
Cell Proliferation
China
Fluorouracil pharmacology
Genes, p16
Humans
Male
Mice
Mice, Nude
Middle Aged
Mitomycin pharmacology
Stomach Neoplasms genetics
Tumor Suppressor Protein p53 genetics
Carcinoma, Signet Ring Cell pathology
Cell Line, Tumor drug effects
Drug Resistance, Multiple
Drug Resistance, Neoplasm
Stomach Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2423
- Volume :
- 46
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- International journal of oncology
- Publication Type :
- Academic Journal
- Accession number :
- 25892440
- Full Text :
- https://doi.org/10.3892/ijo.2015.2966