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Burkholderia metalliresistens sp. nov., a multiple metal-resistant and phosphate-solubilising species isolated from heavy metal-polluted soil in Southeast China.
- Source :
-
Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2015 Jun; Vol. 107 (6), pp. 1591-8. Date of Electronic Publication: 2015 Apr 21. - Publication Year :
- 2015
-
Abstract
- A metal-resistant and phosphate-solubilising bacterium, designated as strain D414(T), was isolated from heavy metal (Pb, Cd, Cu and Zn)-polluted paddy soils at the surrounding area of Dabao Mountain Mine in Southeast China. The minimum inhibitory concentrations of heavy metals for strain D414(T) were 2000 mg L(-1) (Cd), 800 mg L(-1) (Pb), 150 mg L(-1) (Cu) and 2500 mg L(-1) (Zn). The strain possessed plant growth-promoting properties, such as 1-aminocyclopropane-1-carboxylate assimilation, indole production and phosphate solubilisation. Analysis of 16S rRNA gene sequence indicated that the isolate is a member of the genus Burkholderia where strain D414(T) formed a distinct phyletic line with validly described Burkholderia species. Strain D414(T) is closely related to Burkholderia tropica DSM 15359(T), B. bannensis NBRC E25(T) and B. unamae DSM 17197(T), with 98.5, 98.3 and 98.3 % sequence similarities, respectively. Furthermore, less than 34 % DNA-DNA relatedness was detected between strain D414(T) and the type strains of the phylogenetically closest species of Burkholderia. The dominant fatty acids of strain D414(T) were C14:0, C16:0, C17:0 cyclo and C18:1 ω7c. The DNA G+C content was 62.3 ± 0.5 mol%. On the basis of genotypic, phenotypic and phylogenetic data, strain D414(T) represents a novel species, for which the name Burkholderia metalliresistens sp. nov. is proposed, with D414(T) (=CICC 10561(T) = DSM 26823(T)) as the type strain.
- Subjects :
- Bacterial Typing Techniques
Base Composition
Burkholderia genetics
Burkholderia physiology
China
Cluster Analysis
Cytosol chemistry
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
Fatty Acids analysis
Indoles metabolism
Microbial Sensitivity Tests
Microscopy, Electron, Scanning
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Soil Pollutants toxicity
Burkholderia classification
Burkholderia isolation & purification
Drug Resistance, Bacterial
Metals, Heavy toxicity
Phosphates metabolism
Soil Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1572-9699
- Volume :
- 107
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Antonie van Leeuwenhoek
- Publication Type :
- Academic Journal
- Accession number :
- 25896306
- Full Text :
- https://doi.org/10.1007/s10482-015-0453-z