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[CCL19/IL-1beta positive feedback loop involved in the progress of inflammation in rheumatoid arthritis].
- Source :
-
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition [Sichuan Da Xue Xue Bao Yi Xue Ban] 2015 Mar; Vol. 46 (2), pp. 272-5. - Publication Year :
- 2015
-
Abstract
- Objective: To explore the function and mechanism of CCL19 in the pathogenesis of rheumatoid arthritis.<br />Methods: Synovial fibroblasts were collected from 5 cases of rheumatoid arthritis. Peripheral blood mononuclear cells (PBMCs) were obtained from 5 healthy people by Ficoll-Hypaque density gradien centrifugation. The cells were stimulated with IL-1beta, TNF-alpha, IL-17 and other cytokines, and then the expression of CCL19 was detected by RT-PCR. The cells also were treated with different concentration of CCL19, then the expressions of IL-1beta, TNF-alpha were detected by RT-PCR, the expressions of p-ERK, p-p38 were detected by western blot.<br />Results: IL-1beta promoted the CCL19/CCR7 expression in both synovial fibroblasts and PBMCs. CCL19 upregulated the expression of IL-10 in both synovial fibroblasts and PBMCs. The stimulation of CCL19 also increased its receptor CCR7 expression. CCL19 activated p-ERK and p-p38 in PBMCs.<br />Conclusion: The positive feedback loop between CCL19 and IL-1 participate in the development of rheumatoid arthritis.
- Subjects :
- Cells, Cultured
Fibroblasts metabolism
Humans
Interleukin-10 metabolism
Interleukin-17 pharmacology
Leukocytes, Mononuclear metabolism
MAP Kinase Signaling System
Synovial Membrane cytology
Tumor Necrosis Factor-alpha pharmacology
Arthritis, Rheumatoid physiopathology
Chemokine CCL19 metabolism
Inflammation physiopathology
Interleukin-1beta pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1672-173X
- Volume :
- 46
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
- Publication Type :
- Academic Journal
- Accession number :
- 25924444