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MTA2 enhances colony formation and tumor growth of gastric cancer cells through IL-11.
- Source :
-
BMC cancer [BMC Cancer] 2015 May 02; Vol. 15, pp. 343. Date of Electronic Publication: 2015 May 02. - Publication Year :
- 2015
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Abstract
- Background: We have preliminarily reported MTA2 expression in gastric cancer and its biological functions by using knockdown cell models, while the molecular mechanisms of MTA2 in regulating malignant behaviors are still unclear.<br />Methods: MTA2 overexpression models were established by transfection assay in gastric cancer cells BGC-823 and MKN28. Cell proliferation assay, colony formation in soft agar, wound-healing assay and transwell migration assay were performed with MTA2 overexpression and negative control (NC) cells. Subcutaneous xenografts and pulmonary metastasis models by BGC-823/MTA2 and BGC-823/NC cells were used to observe the capacity of growth and metastasis in vivo. Differential gene expression in MTA2 knockdown and overexpression cells was analyzed by microarrays. IL-11, which demonstrated as differential expression in microarray, was detected by real-time PCR, western blot, ELISA and immunohistochemistry staining. Recombinant human IL-11 (rhIL-11) was administrated in cell proliferation and colony formation as rescue assay.<br />Results: The numbers of colonies in soft agar were significantly more in BGC-823/MTA2 and MKN28/MTA2 cells, comparing with those in their NC cells. Capabilities of cell proliferation, wound-healing and cell migration were not significantly changed in MTA2 overexpression cells. The sizes of subcutaneous xenografts and pulmonary metastases of BGC-832/MTA2 cells were significantly larger than those in BGC-823/NC group. Differential expression of IL-11 was identified by genome expression microarray both in MTA2 knockdown and overexpression cells. IL-11 expression was elevated in BGC-823/MTA2 cells, whereas reduced in SGC-7901/shMTA2 cells. Administration of rhIL-11 recovered colony formation capacity of SGC-7901/shMTA2 cells.<br />Conclusions: MTA2 overexpression enhances colony formation and tumor growth of gastric cancer cells, but not plays important role in cancer cell migration and metastasis. IL-11 is one of the downstream effectors of MTA2 in regulating gastric cancer cells growth.
- Subjects :
- Apoptosis genetics
Cell Line, Tumor
Cell Movement genetics
Cell Proliferation genetics
Gene Expression Regulation, Neoplastic
Histone Deacetylases genetics
Humans
Interleukin-11 metabolism
Neoplasm Invasiveness genetics
Neoplasm Invasiveness pathology
Repressor Proteins genetics
Stomach Neoplasms pathology
Cell Transformation, Neoplastic genetics
Histone Deacetylases biosynthesis
Interleukin-11 genetics
Repressor Proteins biosynthesis
Stomach Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2407
- Volume :
- 15
- Database :
- MEDLINE
- Journal :
- BMC cancer
- Publication Type :
- Academic Journal
- Accession number :
- 25929737
- Full Text :
- https://doi.org/10.1186/s12885-015-1366-y