Back to Search
Start Over
Post-infarct treatment with [Pyr(1)]apelin-13 improves myocardial function by increasing neovascularization and overexpression of angiogenic growth factors in rats.
- Source :
-
European journal of pharmacology [Eur J Pharmacol] 2015 Aug 15; Vol. 761, pp. 101-8. Date of Electronic Publication: 2015 May 01. - Publication Year :
- 2015
-
Abstract
- Ischemic heart disease is the leading cause of mortality in the world. Angiogenesis is important for cardiac repair after myocardial infarction (MI) as restores blood supply to the ischemic myocardium and preserves cardiac function. Apelin is a peptide that has been recently shown to potentiate angiogenesis. The aim of this study was to investigate angiogenic effects of [Pyr(1)]apelin-13 in the rat model of post-MI. Male Wistar rats (n=36) were randomly divided into three groups: (1) sham (2) MI and (3) MI treated with [Pyr(1)]apelin-13 (MI+Apel). MI animals were subjected to 30min left anterior descending coronary artery (LAD) ligation and 14 days of reperfusion. Twenty-four hours after LAD ligation, [Pyr(1)]apelin-13 (10nmol/kg/day) was administered i.p. for 5 days. Hemodynamic functions by catheter introduced into the left ventricle (LV), myocardial fibrosis by Masson׳s trichrome staining, gene expression of vascular endothelial growth factor-A (VEGFA), VEGF receptor-2 (Kdr), Ang-1 (angiopoietin-1), Tie2 (tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 2) and eNOS by Real-time polymerase chain reaction (Real-Time PCR) and myocardial angiogenesis by CD31 imunostaining were assessed at day 14 post-MI. Post-infarct treatment with [Pyr(1)]apelin-13 improved LV function and decreased myocardial fibrosis. [Pyr(1)]apelin-13 treatment led to a significant increase in the expression of VEGFA, Kdr, Ang-1, Tie2 and eNOS. Further, treatment with [Pyr(1)]apelin-13 promoted capillary density. [Pyr(1)]apelin-13 has angiogenic and anti-fibrotic activity via formation of new blood vessels and overexpression of VEGFA, Kdr, Ang-1, Tie2 and eNOS in the infarcted myocardium which could in turn repair myocardium and improve LV function.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Angiogenic Proteins genetics
Angiopoietin-1 genetics
Angiopoietin-1 metabolism
Animals
Disease Models, Animal
Fibrosis
Male
Myocardial Infarction genetics
Myocardial Infarction metabolism
Myocardial Infarction pathology
Myocardial Infarction physiopathology
Myocardium pathology
Nitric Oxide Synthase Type III genetics
Nitric Oxide Synthase Type III metabolism
Rats, Wistar
Receptor, TIE-2 genetics
Receptor, TIE-2 metabolism
Recovery of Function
Signal Transduction drug effects
Time Factors
Up-Regulation
Vascular Endothelial Growth Factor A genetics
Vascular Endothelial Growth Factor A metabolism
Vascular Endothelial Growth Factor Receptor-2 genetics
Vascular Endothelial Growth Factor Receptor-2 metabolism
Angiogenic Proteins metabolism
Cardiotonic Agents pharmacology
Intercellular Signaling Peptides and Proteins pharmacology
Myocardial Contraction drug effects
Myocardial Infarction drug therapy
Myocardium metabolism
Neovascularization, Physiologic drug effects
Ventricular Function, Left drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0712
- Volume :
- 761
- Database :
- MEDLINE
- Journal :
- European journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 25936512
- Full Text :
- https://doi.org/10.1016/j.ejphar.2015.04.034