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AIM2 co-immunization favors specific multifunctional CD8(+) T cell induction and ameliorates coxsackievirus B3-induced chronic myocarditis.
AIM2 co-immunization favors specific multifunctional CD8(+) T cell induction and ameliorates coxsackievirus B3-induced chronic myocarditis.
- Source :
-
Antiviral research [Antiviral Res] 2015 Jul; Vol. 119, pp. 68-77. Date of Electronic Publication: 2015 May 05. - Publication Year :
- 2015
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Abstract
- Coxsackievirus B3 (CVB3) infection can cause acute myocarditis and chronic myocarditis, leading to dilated cardiomyopathy (DCM) with no effective therapeutic strategy. Therefore, we investigated the potential of absent in melanoma 2 (AIM2) to enhance the therapeutic efficacy of DNA vaccine against CVB3-induced chronic myocarditis. Mice were infected with CVB3 and then intranasally immunized with chitosan-pcDNA3.1 (mock), chitosan-pAIM2 (CS-pAIM2), chitosan-pVP1 (CS-pVP1), or chitosan-pAIM2 plus chitosan-pVP1 (CS-pAIM2/CS-pVP1) at 7, 21, and 35d. Therapeutic efficacies of various vaccines were evaluated at day 56d. Compared with CS-pVP1 immunization, CS-pAIM2/CS-pVP1 co-immunization significantly increased survival rate, improved cardiac function, as well as decreased myocardial injury and fibrosis, this result indicated that CVB3-induced chronic myocarditis was alleviated. CVB3-specific T lymphocyte proliferation and cytotoxic T lymphocyte responses of the CS-pAIM2/CS-pVP1 co-immunization group were also increased. More interestingly, CS-pAIM2/CS-pVP1 co-immunization could facilitate CVB3-specific multifunctional CD8(+) T cell induction in the intestinal mucosa, and this induction was closely correlated with myocardial scores, this result indicated that CS-pAIM2/CS-pVP1 vaccine exhibits therapeutic efficacy by enhancing multifunctional CD8(+) T cells. This study may represent a novel therapy for CVB3-induced chronic myocarditis.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Administration, Intranasal
Animals
Chitosan
Chronic Disease therapy
Coxsackievirus Infections complications
Cytokines immunology
DNA-Binding Proteins genetics
Disease Models, Animal
Intestinal Mucosa immunology
Mice, Inbred BALB C
Myocarditis virology
Myocardium metabolism
Myocardium ultrastructure
Vaccination
Vaccines, DNA immunology
CD8-Positive T-Lymphocytes immunology
Coxsackievirus Infections immunology
Coxsackievirus Infections therapy
DNA-Binding Proteins immunology
Myocarditis therapy
Vaccines, DNA administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1872-9096
- Volume :
- 119
- Database :
- MEDLINE
- Journal :
- Antiviral research
- Publication Type :
- Academic Journal
- Accession number :
- 25956163
- Full Text :
- https://doi.org/10.1016/j.antiviral.2015.04.015