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Molecular analyses detect natural coinfection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) in serologically negative animals.
- Source :
-
Revista Argentina de microbiologia [Rev Argent Microbiol] 2015 Apr-Jun; Vol. 47 (2), pp. 148-51. Date of Electronic Publication: 2015 May 08. - Publication Year :
- 2015
-
Abstract
- Infection of water buffaloes (Bubalus bubalis) with bovine viral diarrhea viruses (BVDV) has been confirmed in several studies by serological and molecular techniques. In order to determine the presence of persistently infected animals and circulating species and subtypes of BVDV we conducted this study on a buffalo herd, whose habitat was shared with bovine cattle (Bossp.). Our serological results showed a high level of positivity for BVDV-1 and BVDV-2 within the buffalo herd. The molecular analyses of blood samples in serologically negative animals revealed the presence of viral nucleic acid, confirming the existence of persistent infection in the buffaloes. Cloning and sequencing of the 5' UTR of some of these samples revealed the presence of naturally mix-infected buffaloes with at least two different subtypes (1a and 1b), and also with both BVDV species (BVDV-1 and BVDV-2).<br /> (Copyright © 2014 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.)
- Subjects :
- 5' Untranslated Regions genetics
Animals
Antibodies, Viral blood
Argentina epidemiology
Asymptomatic Diseases
Bovine Virus Diarrhea-Mucosal Disease epidemiology
Bovine Virus Diarrhea-Mucosal Disease transmission
Carrier State epidemiology
Carrier State virology
Cattle microbiology
DNA, Viral genetics
Diarrhea Virus 1, Bovine Viral classification
Diarrhea Virus 1, Bovine Viral genetics
Diarrhea Virus 1, Bovine Viral immunology
Diarrhea Virus 2, Bovine Viral classification
Diarrhea Virus 2, Bovine Viral genetics
Diarrhea Virus 2, Bovine Viral immunology
Disease Reservoirs
False Negative Reactions
Host Specificity
Pestivirus Infections epidemiology
Pestivirus Infections transmission
Pestivirus Infections virology
Phylogeny
RNA, Viral blood
Species Specificity
Viremia epidemiology
Viremia virology
Buffaloes virology
Carrier State veterinary
Coinfection virology
Diarrhea Virus 1, Bovine Viral isolation & purification
Diarrhea Virus 2, Bovine Viral isolation & purification
Pestivirus Infections veterinary
Viremia veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 0325-7541
- Volume :
- 47
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Revista Argentina de microbiologia
- Publication Type :
- Academic Journal
- Accession number :
- 25962538
- Full Text :
- https://doi.org/10.1016/j.ram.2015.03.001