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In oesophageal squamous cells, nitric oxide causes S-nitrosylation of Akt and blocks SOX2 (sex determining region Y-box 2) expression.
- Source :
-
Gut [Gut] 2016 Sep; Vol. 65 (9), pp. 1416-26. Date of Electronic Publication: 2015 May 18. - Publication Year :
- 2016
-
Abstract
- Objective: Barrett's metaplasia might develop if GORD causes oesophageal squamous cells to convert into columnar cells. Acid and bile exposures upregulate columnar differentiation genes like CDX2 in oesophageal squamous cells, but it is not known if such exposures downregulate squamous differentiation genes like SOX2. In addition to acid and bile, patients with GORD also have high oesophageal concentrations of nitric oxide (NO). This study aims to determine how acid, bile salts and NO affect genes that influence oesophageal cell phenotype.<br />Design: Oesophageal squamous cells from patients with Barrett's oesophagus were exposed to acidic bile salts or NOC-9 (an NO donor). SOX2, p63 (squamous transcription factor) and CDX2 mRNAs were measured by quantitative RT-PCR. SOX2 and its regulatory Akt pathway proteins were evaluated by western blotting. S-nitrosylation by NO was blocked by dithiothreitol. Immunohistochemistry for SOX2 was performed on the oesophagus of rats with surgically induced GORD which were fed diets with and without nitrite supplementation.<br />Results: In oesophageal squamous cells, NO profoundly decreased SOX2 protein and caused a significantly greater decrease in SOX2 mRNA than did acidic bile salts. NO also decreased p63 and increased CDX2 expression. NO caused S-nitrosylation of Akt, blocking its phosphorylation. Akt pathway inhibition by LY294002 or Akt siRNA reduced SOX2 mRNA. Rats fed with nitrite-supplemented diets exhibited weaker SOX2 oesophageal staining than rats fed with normal diets.<br />Conclusions: In oesophageal squamous cells, NO blocks SOX2 expression through Akt S-nitrosylation. NO also increases CDX2 and decreases p63 expression. By triggering molecular events preventing squamous differentiation while promoting intestinal differentiation, NO might contribute to Barrett's pathogenesis.<br /> (Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/)
- Subjects :
- Animals
Bile Acids and Salts metabolism
Cell Differentiation
Cell Line
Disease Models, Animal
Esophagus pathology
Humans
Male
RNA, Messenger metabolism
Rats
Signal Transduction physiology
Barrett Esophagus etiology
Barrett Esophagus metabolism
Barrett Esophagus pathology
CDX2 Transcription Factor metabolism
Epithelial Cells metabolism
Epithelial Cells pathology
Gastroesophageal Reflux complications
Gastroesophageal Reflux metabolism
Gastroesophageal Reflux pathology
Nitric Oxide metabolism
SOXB1 Transcription Factors metabolism
Triazenes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1468-3288
- Volume :
- 65
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Gut
- Publication Type :
- Academic Journal
- Accession number :
- 25986942
- Full Text :
- https://doi.org/10.1136/gutjnl-2015-309272