A rapid and simple method to detect ESBL in Enterobacter cloacae based on MIC of cefepime.

Authors :: Bastos Mde S
Menegucci TC
Moreira RR
Garcia LB
Cardoso CL
Tognim MC
Source :: Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2015 Mar-Apr; Vol. 48 (2), pp. 208-11.
Publication Year :: 2015
Abstract: Introduction: The aim of this study was to identify a rapid and simple phenotypic method for extended-spectrum β-lactamase (ESBL) detection in Enterobacter cloacae.<br />Methods: A total of 79 consecutive, non-repeated samples of E. cloacae were evaluated. Four phenotypic methods were applied for ESBL detection, results were compared to multiplex polymerase chain reaction (PCR) as the gold standard reference method: 1) ceftazidime and cefotaxime disks with and without clavulanate, both with boronic acid added; 2) disk approximation using cefepime and amoxicillin/clavulanate; 3) ESBL screening by minimum inhibitory concentration (MIC) ≥ 16µg/mL and 4) by MIC ≥ 2µg/mL for cefepime.<br />Results: Method 4 showed the best combination of sensitivity (100%) and specificity (94%).<br />Conclusions: MIC ≥ 2µg/mL for cefepime would be very useful for the phenotypic detection of ESBL in samples of E. cloacae.

Subjects :: Adult
Anti-Bacterial Agents pharmacology
Cefepime
Cephalosporins pharmacology
Enterobacter cloacae drug effects
Humans
Intensive Care Units
Microbial Sensitivity Tests methods
Phenotype
Prospective Studies
Sensitivity and Specificity
beta-Lactamases metabolism
Enterobacter cloacae enzymology
beta-Lactamases analysis

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