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Rapid Point-of-Contact Tool for Mapping and Integrated Surveillance of Wuchereria bancrofti and Onchocerca volvulus Infection.
- Source :
-
Clinical and vaccine immunology : CVI [Clin Vaccine Immunol] 2015 Aug; Vol. 22 (8), pp. 896-901. Date of Electronic Publication: 2015 May 27. - Publication Year :
- 2015
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Abstract
- Elimination programs for Wuchereria bancrofti and Onchocerca volvulus are in critical need of sensitive, specific, and point-of-contact (POC) tools that can be used for surveillance years beyond cessation of mass drug administration when infection intensities are low. Previously, Wb123 and Ov16 were identified individually as potential filarial antigens for an antibody-based POC test. The present study compares single-antigen Wb123- and Ov16-based POC tests with an integrated configuration to detect antibodies to Wb123 and Ov16 simultaneously. Wb123 and Ov16 isolates were striped onto lateral flow strips containing anti-IgG4. Sera from W. bancrofti-, O. volvulus-, and other helminth-infected or -uninfected individuals were added to the strips with buffer. Strips were read for the appearance of a positive or negative test line for both antigens at 20 min and following drying. Sensitivity, specificity, and predictive values were calculated for the single-antigen and biplex strips. Single and biplex lateral flow strips showed nearly identical results, with >90% sensitivity for Ov16 and >92% sensitivity for Wb123. Overall specificities for the single and biplex tests were 98% and 96% for Ov16 and Wb123, respectively. Biplex tests performed as well as the single-antigen tests regardless of the intensity of patient IgG4 response. The high sensitivity and specificity make these new biplex tests extremely useful for POC long-term surveillance following mass drug administration in Africa that should reduce time and cost in areas where bancroftian filariasis and onchocerciasis are coendemic.<br /> (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)
- Subjects :
- Africa
Animals
Antibodies, Helminth blood
Antigens, Helminth immunology
Chromatography, Affinity methods
Elephantiasis, Filarial epidemiology
Humans
Onchocerciasis epidemiology
Predictive Value of Tests
Sensitivity and Specificity
Time Factors
Diagnostic Tests, Routine methods
Elephantiasis, Filarial diagnosis
Epidemiological Monitoring
Onchocerca volvulus isolation & purification
Onchocerciasis diagnosis
Point-of-Care Systems
Wuchereria bancrofti isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1556-679X
- Volume :
- 22
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Clinical and vaccine immunology : CVI
- Publication Type :
- Academic Journal
- Accession number :
- 26018537
- Full Text :
- https://doi.org/10.1128/CVI.00227-15