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TLR1 expression in mouse brain was increased in a KA-induced seizure model.
- Source :
-
Inflammation research : official journal of the European Histamine Research Society ... [et al.] [Inflamm Res] 2015 Jul; Vol. 64 (7), pp. 487-95. Date of Electronic Publication: 2015 May 29. - Publication Year :
- 2015
-
Abstract
- Objective: Toll-like receptors (TLRs) that mediate inflammatory responses play an important role in epilepsy; however, whether TLR1 is also involved in epileptogenesis remains unclear. Thus, in this study, we investigated the extent and pattern of TLR1 expression in epileptic tissues.<br />Methods: One-hundred and thirty-two mice were intra-cerebroventricularly injected with PBS or kainic acid (KA) and were examined at 1, 3, 8 and 24 h. The expression pattern and distribution of TLR1 were examined by reverse-transcriptase polymerase chain reaction (RT-PCR), western blot analysis and immunohistochemistry staining.<br />Results: The mRNA and protein levels of TLR1 were significantly upregulated in the hippocampus and temporal cortex of epileptic mice compared with those of controls. TLR1 expression was increased as early as 1 h following KA treatment and peaked at 8 and 24 h. Immunohistochemistry staining demonstrated that TLR1 was distributed in the CA1-3, dentate gyrus and hilus regions of the hippocampus and different cortical regions. Immunofluorescent staining further revealed that TLR1 was primarily expressed in the neurons, microglia, and astrocytes of epileptogenic tissue.<br />Significance: These results demonstrate that cortical and hippocampal sub-regional expression of TLR1 is altered during epileptogenesis in a time- and location-specific manner, suggesting a close association with the process of epilepsy.
- Subjects :
- Animals
Astrocytes drug effects
Astrocytes metabolism
CA1 Region, Hippocampal drug effects
CA1 Region, Hippocampal metabolism
Dentate Gyrus drug effects
Dentate Gyrus metabolism
Epilepsy, Temporal Lobe chemically induced
Epilepsy, Temporal Lobe genetics
Immunohistochemistry
Injections, Intraventricular
Mice
Mice, Inbred C57BL
Microglia drug effects
Microglia metabolism
Neurons drug effects
Neurons metabolism
Polymerase Chain Reaction
RNA, Messenger biosynthesis
RNA, Messenger genetics
Toll-Like Receptor 1 genetics
Up-Regulation drug effects
Brain Chemistry genetics
Excitatory Amino Acid Agonists
Kainic Acid
Seizures chemically induced
Seizures genetics
Toll-Like Receptor 1 biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1420-908X
- Volume :
- 64
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Inflammation research : official journal of the European Histamine Research Society ... [et al.]
- Publication Type :
- Academic Journal
- Accession number :
- 26021825
- Full Text :
- https://doi.org/10.1007/s00011-015-0828-7