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The interaction between nitrogen availability and auxin, cytokinin, and strigolactone in the control of shoot branching in rice (Oryza sativa L.).
- Source :
-
Plant cell reports [Plant Cell Rep] 2015 Sep; Vol. 34 (9), pp. 1647-62. Date of Electronic Publication: 2015 May 30. - Publication Year :
- 2015
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Abstract
- Key Message: Nitrogen availability and cytokinin could promote shoot branching in rice, whereas auxin and strigolactone inhibited it. The interaction between nitrogen availability and the three hormones is discussed. Rice shoot branching is strongly affected by nitrogen availability and the plant hormones auxin, cytokinin, and strigolactone; however, the interaction of them in the regulation of rice shoot branching remains a subject of debate. In the present study, nitrogen and the three hormones were used to regulate rice tiller bud growth in the indica rice variety Yangdao 6. Both nitrogen and CK promoted shoot branching in rice, whereas auxin and SL inhibited it. We used HPLC to determine the amounts of endogenous IAA and CK, and we used quantitative real-time PCR analysis to quantify the expression levels of several genes. Nitrogen enhanced the amount of CK by promoting the expression levels of OsIPTs in nodes. In addition, both nitrogen and CK downregulated the expression of genes related to SL synthesis in root and nodes, implying that the inhibition of SL synthesis by nitrogen may occur at least partially through the CK pathway. SL did not significantly reduce the amount of CK or the expression levels of OsIPT genes, but it did significantly reduce the amount of auxin and the auxin transport capacity in nodes. Auxin itself inhibited CK synthesis and promoted SL synthesis in nodes rather than in roots. Furthermore, we found that CK and SL quickly reduced and increased the expression of FC1 in buds, respectively, implying that FC1 might be a common target for the CK and SL pathways. Nitrogen and auxin delayed expression change patterns of FC1, potentially by changing the downstream signals for CK and SL.
- Subjects :
- Benzyl Compounds
Biosynthetic Pathways drug effects
Biosynthetic Pathways genetics
Gene Expression Regulation, Plant drug effects
Genes, Plant
Kinetin pharmacology
Naphthaleneacetic Acids
Oryza drug effects
Oryza genetics
Plant Growth Regulators pharmacology
Plant Proteins genetics
Plant Proteins metabolism
Plant Shoots drug effects
Purines
Cytokinins pharmacology
Indoleacetic Acids pharmacology
Lactones pharmacology
Nitrogen pharmacology
Oryza growth & development
Plant Shoots growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1432-203X
- Volume :
- 34
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Plant cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 26024762
- Full Text :
- https://doi.org/10.1007/s00299-015-1815-8