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Purification of TEM-1 beta-lactamase by immunoaffinity chromatography.

Authors :
Bibi E
Source :
The Biochemical journal [Biochem J] 1989 Oct 01; Vol. 263 (1), pp. 309-11.
Publication Year :
1989

Abstract

A monoclonal antibody prepared against TEM-1 beta-lactamase was found to compete with penicillins and cephalosporins for binding to the enzyme. The purified antibody preparation was linked to Sepharose 4B and used for immunoaffinity-chromatography purification of TEM-1 beta-lactamase. Elution with either benzylpenicillin or cloxacillin yielded a highly purified, concentrated and active enzyme preparation.

Subjects

Subjects :
Antibodies, Monoclonal
Binding, Competitive
Chromatography, Affinity methods
Cloxacillin isolation & purification
Electrophoresis, Polyacrylamide Gel
Penicillin G isolation & purification
beta-Lactamases isolation & purification

Details

Language :
English
ISSN :
0264-6021
Volume :
263
Issue :
1
Database :
MEDLINE
Journal :
The Biochemical journal
Publication Type :
Academic Journal
Accession number :
2604704
Full Text :
https://doi.org/10.1042/bj2630309
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