Back to Search
Start Over
Analysis of Exhaled Breath Volatile Organic Compounds in Inflammatory Bowel Disease: A Pilot Study.
- Source :
-
Journal of Crohn's & colitis [J Crohns Colitis] 2015 Sep; Vol. 9 (9), pp. 731-7. Date of Electronic Publication: 2015 Jun 12. - Publication Year :
- 2015
-
Abstract
- Background and Aims: Distinguishing between the inflammatory bowel diseases [IBD], Crohn's disease [CD] and ulcerative colitis [UC], is important for determining management and prognosis. Selected ion flow tube mass spectrometry [SIFT-MS] may be used to analyse volatile organic compounds [VOCs] in exhaled breath: these may be altered in disease states, and distinguishing breath VOC profiles can be identified. The aim of this pilot study was to identify, quantify, and analyse VOCs present in the breath of IBD patients and controls, potentially providing insights into disease pathogenesis and complementing current diagnostic algorithms.<br />Methods: SIFT-MS breath profiling of 56 individuals [20 UC, 18 CD, and 18 healthy controls] was undertaken. Multivariate analysis included principal components analysis and partial least squares discriminant analysis with orthogonal signal correction [OSC-PLS-DA]. Receiver operating characteristic [ROC] analysis was performed for each comparative analysis using statistically significant VOCs.<br />Results: OSC-PLS-DA modelling was able to distinguish both CD and UC from healthy controls and from one other with good sensitivity and specificity. ROC analysis using combinations of statistically significant VOCs [dimethyl sulphide, hydrogen sulphide, hydrogen cyanide, ammonia, butanal, and nonanal] gave integrated areas under the curve of 0.86 [CD vs healthy controls], 0.74 [UC vs healthy controls], and 0.83 [CD vs UC].<br />Conclusions: Exhaled breath VOC profiling was able to distinguish IBD patients from controls, as well as to separate UC from CD, using both multivariate and univariate statistical techniques.<br /> (Copyright © 2015 European Crohn’s and Colitis Organisation (ECCO). Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
- Subjects :
- Adult
Aged
Aged, 80 and over
Biomarkers metabolism
Breath Tests
Case-Control Studies
Colitis, Ulcerative metabolism
Crohn Disease metabolism
Diagnosis, Differential
Discriminant Analysis
Female
Humans
Male
Mass Spectrometry methods
Middle Aged
Models, Statistical
Pilot Projects
Principal Component Analysis
ROC Curve
Sensitivity and Specificity
Colitis, Ulcerative diagnosis
Crohn Disease diagnosis
Volatile Organic Compounds metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1876-4479
- Volume :
- 9
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of Crohn's & colitis
- Publication Type :
- Academic Journal
- Accession number :
- 26071410
- Full Text :
- https://doi.org/10.1093/ecco-jcc/jjv102