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Evaluation of Bar, Barnase, and Barstar recombinant proteins expressed in genetically engineered Brassica juncea (Indian mustard) for potential risks of food allergy using bioinformatics and literature searches.
- Source :
-
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2015 Sep; Vol. 83, pp. 93-102. Date of Electronic Publication: 2015 Jun 14. - Publication Year :
- 2015
-
Abstract
- The potential allergenicity of Bar, Barnase, and Barstar recombinant proteins expressed in genetically engineered mustard for pollination control in plant breeding was evaluated for regulatory review. To evaluate the potential allergenicity of the Bar, Barnase and Barstar proteins amino acid sequence comparisons were made to those of known and putative allergens, and search for published evidence to the sources of the genes using the AllergenOnline.org database. Initial comparisons in 2012 were performed with version 12 by methods recommended by the Codex Alimentarius Commission and the Indian Council of Medical Research, Government of India. Searches were repeated with version 15 in 2015. A literature search was performed using PubMed to identify reports of allergy associated with the sources of the three transgenes. Potential open reading frames at the DNA insertion site were evaluated for matches to allergens. No significant sequence identity matches were identified with Bar, Barnase or Barstar proteins or potential fusion peptides at the genomic-insert junctions compared to known allergens. No references were identified that associated the sources of the genes with allergy. Based on these results we conclude that the Bar, Barnase and Barstar proteins are unlikely to present any significant risk of food allergy to consumers.<br /> (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Subjects :
- Acetyltransferases adverse effects
Acetyltransferases chemistry
Acetyltransferases genetics
Acetyltransferases metabolism
Allergens chemistry
Allergens genetics
Allergens metabolism
Amino Acid Sequence
Antigens, Plant adverse effects
Antigens, Plant chemistry
Antigens, Plant genetics
Antigens, Plant metabolism
Bacillus enzymology
Bacterial Proteins chemistry
Bacterial Proteins genetics
Bacterial Proteins metabolism
Computational Biology
Databases, Protein
Dietary Proteins adverse effects
Dietary Proteins chemistry
Dietary Proteins metabolism
Food Hypersensitivity etiology
Food Hypersensitivity prevention & control
Humans
India
Mustard Plant enzymology
Mustard Plant genetics
Plants, Genetically Modified enzymology
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Pollination
Recombinant Proteins adverse effects
Recombinant Proteins chemistry
Recombinant Proteins metabolism
Ribonucleases adverse effects
Ribonucleases chemistry
Ribonucleases genetics
Ribonucleases metabolism
Risk Assessment
Seeds enzymology
Seeds genetics
Sequence Alignment
Streptomyces enzymology
Allergens adverse effects
Bacterial Proteins adverse effects
Food, Genetically Modified adverse effects
Models, Molecular
Mustard Plant metabolism
Seeds metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6351
- Volume :
- 83
- Database :
- MEDLINE
- Journal :
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
- Publication Type :
- Academic Journal
- Accession number :
- 26079618
- Full Text :
- https://doi.org/10.1016/j.fct.2015.06.003