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Tissue landscape alters adjacent cell fates during Drosophila egg development.
- Source :
-
Nature communications [Nat Commun] 2015 Jun 17; Vol. 6, pp. 7356. Date of Electronic Publication: 2015 Jun 17. - Publication Year :
- 2015
-
Abstract
- Extracellular signalling molecules control many biological processes, but the influence of tissue architecture on the local concentrations of these factors is unclear. Here we examine this issue in the Drosophila egg chamber, where two anterior cells secrete Unpaired (Upd) to activate Signal transducer and activator of transcription (STAT) signalling in the epithelium. High STAT signalling promotes cell motility. Genetic analysis shows that all cells near the Upd source can respond. However, using upright imaging, we show surprising asymmetries in STAT activation patterns, suggesting that some cells experience different Upd levels than predicted by their location. We develop a three-dimensional mathematical model to characterize the spatio-temporal distribution of the activator. Simulations show that irregular tissue domains can produce asymmetric distributions of Upd, consistent with results in vivo. Mutant analysis substantiates this idea. We conclude that cellular landscape can heavily influence the effect of diffusible activators and should be more widely considered.
- Subjects :
- Animals
CCAAT-Enhancer-Binding Proteins genetics
CCAAT-Enhancer-Binding Proteins metabolism
Drosophila embryology
Drosophila Proteins genetics
Female
Male
Models, Biological
Ovum growth & development
Receptors, Notch metabolism
Drosophila physiology
Drosophila Proteins metabolism
STAT Transcription Factors metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 26082073
- Full Text :
- https://doi.org/10.1038/ncomms8356