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ROS-induced oxidative stress and apoptosis-like event directly affect the cell viability of cryopreserved embryogenic callus in Agapanthus praecox.
- Source :
-
Plant cell reports [Plant Cell Rep] 2015 Sep; Vol. 34 (9), pp. 1499-513. Date of Electronic Publication: 2015 Jun 24. - Publication Year :
- 2015
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Abstract
- Key Message: Oxidative stress and apoptosis-like programmed cell death, induced in part by H 2 O 2 , are two key factors that damage cells during plant cryopreservation. Their inhibition can improve cell viability. We hypothesized that oxidative stress and apoptosis-like event induced by ROS seriously impact plant cell viability during cryopreservation. This study documented changes in cell morphology and ultrastructure, and detected dynamic changes in ROS components (O 2 (·-) , H2O2 and OH·), antioxidant systems, and programmed cell death (PCD) events during embryonic callus cryopreservation of Agapanthus praecox. Plasmolysis, organelle ultrastructure changes, and increases in malondialdehyde (a membrane lipid peroxidation product) suggested that oxidative damage and PCD events occurred at several early cryopreservation steps. PCD events including autophagy, apoptosis-like, and necrosis also occurred at later stages of cryopreservation, and most were apoptosis. H2O2 is the most important ROS molecule mediating oxidative damage and affecting cell viability, and catalase and AsA-GSH cycle are involved in scavenging the intracellular H2O2 and protecting the cells against stress damage in the whole process. Gene expression studies verified changes of antioxidant system and PCD-related genes at the main steps of the cryopreservation process that correlated with improved cell viability. Reducing oxidative stress or inhibition of apoptosis-like event by deactivating proteases improved cryopreserved cell viability from 49.14 to 86.85 % and 89.91 %, respectively. These results verify our model of ROS-induced oxidative stress and apoptosis-like event in plant cryopreservation. This study provided a novel insight into cell stress response mechanisms in cryopreservation.
- Subjects :
- Antioxidants metabolism
Autophagy drug effects
Cell Shape drug effects
Cell Survival drug effects
Gene Expression Profiling
Gene Expression Regulation, Plant drug effects
In Situ Nick-End Labeling
Liliaceae genetics
Liliaceae ultrastructure
Lipid Peroxidation drug effects
Malondialdehyde metabolism
Real-Time Polymerase Chain Reaction
Seeds drug effects
Seeds ultrastructure
Apoptosis drug effects
Cryopreservation
Liliaceae cytology
Liliaceae embryology
Oxidative Stress drug effects
Reactive Oxygen Species toxicity
Seeds cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-203X
- Volume :
- 34
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Plant cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 26104871
- Full Text :
- https://doi.org/10.1007/s00299-015-1802-0