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Effects of a Dissostichus mawsoni-CaM recombinant proteins feed additive on the juvenile orange-spotted grouper (Epinephelus coioides) under the acute low temperature challenge.

Authors :
Luo SW
Wang WN
Cai L
Qi ZH
Wang C
Liu Y
Peng CL
Chen LB
Source :
Fish physiology and biochemistry [Fish Physiol Biochem] 2015 Oct; Vol. 41 (5), pp. 1345-58. Date of Electronic Publication: 2015 Jun 30.
Publication Year :
2015

Abstract

The effects of Dissostichus mawsoni-Calmodulin (Dm-CaM) on growth performance, enzyme activities, respiratory burst, MDA level and immune-related gene expressions of the orange-spotted grouper (Epinephelus coioides) exposed to the acute low temperature stress were evaluated. The commercial diet supplemented with Dm-CaM protein was fed to the groupers for 6 weeks. No significant difference was observed in the specific growth rates, weight gains and survivals. After the feeding trial, the groupers were exposed to acute low temperature challenge. The groupers fed with Dm-CaM additive diet showed a significant decrease in the respiratory burst activity, while the blood cell number increased significantly at 25 °C by comparing with the control and additive control group. The enzymatic activity of SOD, ACP and ALP increased significantly in Dm-CaM additive group, while MDA level maintained stable with the lowest value. qRT-PCR analysis indicated that the up-regulated transcript expressions of CaM, C3, SOD2, LysC and HSPA4 were observed in Dm-CaM additive group. These results indicated that Dm-CaM additive diet may regulate the grouper immune response to the acute low temperature challenge.

Details

Language :
English
ISSN :
1573-5168
Volume :
41
Issue :
5
Database :
MEDLINE
Journal :
Fish physiology and biochemistry
Publication Type :
Academic Journal
Accession number :
26122279
Full Text :
https://doi.org/10.1007/s10695-015-0090-5