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In vivo biocompatibility and in vitro efficacy of antimicrobial gendine-coated central catheters.
- Source :
-
Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2015 Sep; Vol. 59 (9), pp. 5611-8. Date of Electronic Publication: 2015 Jun 29. - Publication Year :
- 2015
-
Abstract
- Antimicrobial peripherally inserted central catheters (PICCs) might reduce the incidence of central line-associated bloodstream infections (CLABSI). We tested the biocompatibility of a novel gendine-coated (combination of chlorhexidine [CHX] and gentian violet [GV]) PICC in a rabbit intravascular model and tested antimicrobial efficacy in comparison with commercially available minocycline/rifampin (M/R)- and CHX-treated PICCs in an in vitro biofilm colonization model. Gendine-coated and uncoated control PICCs were inserted in the jugular veins of rabbits for 4 days. Histopathological analysis was performed at the end of the 4-day period, and circulating levels of CHX and GV in the blood were measured at different time points using liquid chromatography-mass spectrometry. The antimicrobial efficacy of the PICCs was tested following simulated intravascular indwells of 24 h and 1 week against clinical isolates of methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, Pseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii, Enterobacter cloacae, Candida albicans, and Candida glabrata. Rabbits implanted with gendine-coated PICCs exhibited reduced levels of thrombosis and inflammation compared to those of the rabbits with uncoated controls. No GV was detected in blood samples over the entire study period, and trace concentrations of CHX were detected. The gendine-coated PICCs completely prevented the adherence of all pathogens from 24 h to 1 week (P ≤ 0.001), while M/R-treated, CHX-treated, and control PICCs did not. Gendine-coated PICCs were highly effective in preventing biofilm formation of multidrug-resistant pathogenic bacteria and fungi. Gendine-coated PICCs were biocompatible in an intravascular setting. Further, the pharmacokinetic testing established that acute systemic exposures of CHX and GV from the gendine-coated catheters were well within safe levels.<br /> (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)
- Subjects :
- Acinetobacter baumannii drug effects
Animals
Anti-Infective Agents adverse effects
Biofilms drug effects
Candida albicans drug effects
Candida glabrata drug effects
Enterobacter cloacae drug effects
Female
Methicillin-Resistant Staphylococcus aureus drug effects
Microbial Sensitivity Tests
Minocycline adverse effects
Minocycline pharmacology
Pseudomonas aeruginosa drug effects
Rabbits
Rifampin adverse effects
Rifampin pharmacology
Vancomycin-Resistant Enterococci drug effects
Anti-Infective Agents pharmacology
Catheters, Indwelling microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1098-6596
- Volume :
- 59
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Antimicrobial agents and chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 26124171
- Full Text :
- https://doi.org/10.1128/AAC.00834-15