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Multimodal imaging reveals temporal and spatial microglia and matrix metalloproteinase activity after experimental stroke.
- Source :
-
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism [J Cereb Blood Flow Metab] 2015 Nov; Vol. 35 (11), pp. 1711-21. Date of Electronic Publication: 2015 Jul 01. - Publication Year :
- 2015
-
Abstract
- Stroke is the most common cause of death and disability from neurologic disease in humans. Activation of microglia and matrix metalloproteinases (MMPs) is involved in positively and negatively affecting stroke outcome. Novel, noninvasive, multimodal imaging methods visualizing microglial and MMP alterations were employed. The spatio-temporal dynamics of these parameters were studied in relation to blood flow changes. Micro positron emission tomography (μPET) using [(18)F]BR-351 showed MMP activity within the first days after transient middle cerebral artery occlusion (tMCAo), followed by increased [(18)F]DPA-714 uptake as a marker for microglia activation with a maximum at 14 days after tMCAo. The inflammatory response was spatially located in the infarct core and in adjacent (penumbral) tissue. For the first time, multimodal imaging based on PET, single photon emission computed tomography, and magnetic resonance imaging revealed insight into the spatio-temporal distribution of critical parameters of poststroke inflammation. This allows further evaluation of novel treatment paradigms targeting the postischemic inflammation.
- Subjects :
- Animals
Immunohistochemistry
Infarction, Middle Cerebral Artery diagnostic imaging
Infarction, Middle Cerebral Artery metabolism
Magnetic Resonance Imaging
Mice
Mice, Inbred C57BL
Microglia pathology
Positron-Emission Tomography
Pyrazoles
Pyrimidines
Radiopharmaceuticals
Stroke pathology
Technetium Tc 99m Exametazime
Tomography, Emission-Computed, Single-Photon
Matrix Metalloproteinases metabolism
Microglia diagnostic imaging
Multimodal Imaging methods
Neuroimaging methods
Stroke diagnostic imaging
Stroke enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1559-7016
- Volume :
- 35
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 26126867
- Full Text :
- https://doi.org/10.1038/jcbfm.2015.149