Scaffold electrodes based on thioctic acid-capped gold nanoparticles coordinated Alcohol Dehydrogenase and Azure A films for high performance biosensor.

Authors :: Gómez-Anquela C
García-Mendiola T
Abad JM
Pita M
Pariente F
Lorenzo E
Source :: Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2015 Dec; Vol. 106 (Pt B), pp. 335-42. Date of Electronic Publication: 2015 Jun 30.
Publication Year :: 2015
Abstract: Nanometric size gold nanoparticles capped with thiotic acid are used to coordinate with the Zn (II) present in the catalytic center of Alcohol Dehydrogenase (ADH). In combination with the NADH oxidation molecular catalyst Azure A, electrografted onto carbon screen-printed electrodes, they are used as scaffold electrodes for the construction of a very efficient ethanol biosensor. The final biosensing device exhibits a highly efficient ethanol oxidation with low overpotential of -0.25 V besides a very good analytical performance with a detection limit of 0.14±0.01 μM and a stable response for more than one month.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)

Subjects :: Alcohol Dehydrogenase chemistry
Catalysis
Equipment Design
Food Analysis instrumentation
Food Analysis methods
Gold
Metal Nanoparticles chemistry
Oxidation-Reduction
Thioctic Acid chemistry
Wine analysis
Azure Stains chemistry
Biosensing Techniques instrumentation
Biosensing Techniques methods
Electrodes
Ethanol analysis

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