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Salix purpurea Stimulates the Expression of Specific Bacterial Xenobiotic Degradation Genes in a Soil Contaminated with Hydrocarbons.
- Source :
-
PloS one [PLoS One] 2015 Jul 10; Vol. 10 (7), pp. e0132062. Date of Electronic Publication: 2015 Jul 10 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- The objectives of this study were to uncover Salix purpurea-microbe xenobiotic degradation systems that could be harnessed in rhizoremediation, and to identify microorganisms that are likely involved in these partnerships. To do so, we tested S. purpurea's ability to stimulate the expression of 10 marker microbial oxygenase genes in a soil contaminated with hydrocarbons. In what appeared to be a detoxification rhizosphere effect, transcripts encoding for alkane 1-monooxygenases, cytochrome P450 monooxygenases, laccase/polyphenol oxidases, and biphenyl 2,3-dioxygenase small subunits were significantly more abundant in the vicinity of the plant's roots than in bulk soil. This gene expression induction is consistent with willows' known rhizoremediation capabilities, and suggests the existence of S. purpurea-microbe systems that target many organic contaminants of interest (i.e. C4-C16 alkanes, fluoranthene, anthracene, benzo(a)pyrene, biphenyl, polychlorinated biphenyls). An enhanced expression of the 4 genes was also observed within the bacterial orders Actinomycetales, Rhodospirillales, Burkholderiales, Alteromonadales, Solirubrobacterales, Caulobacterales, and Rhizobiales, which suggest that members of these taxa are active participants in the exposed partnerships. Although the expression of the other 6 marker genes did not appear to be stimulated by the plant at the community level, signs of additional systems that rest on their expression by members of the orders Solirubrobacterales, Sphingomonadales, Actinomycetales, and Sphingobacteriales were observed. Our study presents the first transcriptomics-based identification of microbes whose xenobiotic degradation activity in soil appears stimulated by a plant. It paints a portrait that contrasts with the current views on these consortia's composition, and opens the door for the development of laboratory test models geared towards the identification of root exudate characteristics that limit the efficiency of current willow-based rhizoremediation applications.
- Subjects :
- Actinomycetales enzymology
Actinomycetales genetics
Alteromonadaceae enzymology
Alteromonadaceae genetics
Bacterial Proteins genetics
Bacterial Proteins metabolism
Biodegradation, Environmental
Burkholderiaceae enzymology
Burkholderiaceae genetics
Caulobacteraceae enzymology
Caulobacteraceae genetics
Cytochrome P-450 CYP4A genetics
Cytochrome P-450 CYP4A metabolism
Cytochrome P-450 Enzyme System genetics
Cytochrome P-450 Enzyme System metabolism
Gene Expression Regulation, Bacterial
Genes, Bacterial
Iron-Sulfur Proteins genetics
Iron-Sulfur Proteins metabolism
Laccase genetics
Laccase metabolism
Metabolic Networks and Pathways
Oxygenases genetics
Oxygenases metabolism
Rhizobiaceae enzymology
Rhizobiaceae genetics
Rhodospirillales enzymology
Rhodospirillales genetics
Xenobiotics
Petroleum Pollution analysis
Salix physiology
Soil Pollutants analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 26161539
- Full Text :
- https://doi.org/10.1371/journal.pone.0132062