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Choroidal mast cells in retinal pathology: a potential target for intervention.
- Source :
-
The American journal of pathology [Am J Pathol] 2015 Aug; Vol. 185 (8), pp. 2083-95. Date of Electronic Publication: 2015 Jul 09. - Publication Year :
- 2015
-
Abstract
- Mast cells are important in the initiation of ocular inflammation, but the consequences of mast cell degranulation on ocular pathology remain uncharacterized. We induced mast cell degranulation by local subconjunctival injection of compound 48/80. Initial degranulation of mast cells was observed in the choroid 15 minutes after the injection and increased up to 3 hours after injection. Clinical signs of anterior segment inflammation paralleled mast cell degranulation. With the use of optical coherence tomography, dilation of choroidal vessels and serous retinal detachments (SRDs) were observed and confirmed by histology. Subconjunctival injection of disodium cromoglycate significantly reduced the rate of SRDs, demonstrating the involvement of mast cell degranulation in posterior segment disorders. The infiltration of polymorphonuclear and macrophage cells was associated with increased ocular media concentrations of tumor necrosis factor-α, CXCL1, IL-6, IL-5, chemokine ligand 2, and IL-1β. Analysis of the amounts of vascular endothelial growth factor and IL-18 showed an opposite evolution of vascular endothelial growth factor compared with IL-18 concentrations, suggesting that they regulate each other's production. These findings suggest that the local degranulation of ocular mast cells provoked acute ocular inflammation, dilation, increased vascular permeability of choroidal vessels, and SRDs. The involvement of mast cells in retinal diseases should be further investigated. The pharmacologic inhibition of mast cell degranulation may be a potential target for intervention.<br /> (Copyright © 2015 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Capillary Permeability drug effects
Chemokines metabolism
Choroid drug effects
Choroid metabolism
Cytokines metabolism
Female
Mast Cells drug effects
Mast Cells metabolism
Rats
Rats, Inbred Lew
Retina drug effects
Retina metabolism
Tomography, Optical Coherence
p-Methoxy-N-methylphenethylamine pharmacology
Cell Degranulation drug effects
Choroid pathology
Mast Cells pathology
Retina pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1525-2191
- Volume :
- 185
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- The American journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 26166807
- Full Text :
- https://doi.org/10.1016/j.ajpath.2015.04.002