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Loss of GSK-3 Causes Abnormal Astrogenesis and Behavior in Mice.
- Source :
-
Molecular neurobiology [Mol Neurobiol] 2016 Aug; Vol. 53 (6), pp. 3954-3966. Date of Electronic Publication: 2015 Jul 17. - Publication Year :
- 2016
-
Abstract
- Altered activity of glycogen synthase kinase-3 (GSK-3) is associated with psychiatric diseases and neurodegenerative diseases. GSK-3 is a key regulator in multiple aspects of neuronal differentiation in the brain. However, little is known about the role of GSK-3 in astrocyte development. To examine the role of GSK-3 in astrocytes, we generated a conditional knockout mouse using a glial fibrillary acidic protein (GFAP)-cre driver, in which the GSK-3 alpha and beta genes are deleted in astrocytes. We found that GFAP-cre-mediated GSK-3 deletion led to a larger brain. The number and size of astrocytes were increased in GSK-3 mutant brains. The levels of GFAP and phospho-STAT3, indicators of astrogenesis, were elevated in GSK-3 mutants. Furthermore, we found upregulation of astrocyte regulatory molecules such as phospho-AKT, phospho-S6, and cyclin D in GSK-3 mutant brains. Finally, GSK-3 mutant mice exhibited aberrant anxiety and social behavior. Our results suggest that GSK-3 plays a significant role in astrocyte development and behavioral control in mice.
- Subjects :
- Animals
Anxiety enzymology
Anxiety pathology
Brain pathology
Caspase 3 metabolism
Cell Count
Cell Proliferation
Gene Deletion
Glial Fibrillary Acidic Protein metabolism
Hypertrophy
Mice
Mice, Mutant Strains
Mutation genetics
RNA, Messenger genetics
RNA, Messenger metabolism
Signal Transduction
Astrocytes enzymology
Astrocytes pathology
Behavior, Animal
Glycogen Synthase Kinase 3 metabolism
Glycogen Synthase Kinase 3 beta metabolism
Neurogenesis
Subjects
Details
- Language :
- English
- ISSN :
- 1559-1182
- Volume :
- 53
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Molecular neurobiology
- Publication Type :
- Academic Journal
- Accession number :
- 26179612
- Full Text :
- https://doi.org/10.1007/s12035-015-9326-8