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Targeting growth factor supply in keratopathy treatment: comparison between maternal peripheral blood and cord blood as sources for the preparation of topical eye drops.
- Source :
-
Blood transfusion = Trasfusione del sangue [Blood Transfus] 2016 Mar; Vol. 14 (2), pp. 145-51. Date of Electronic Publication: 2015 Jul 09. - Publication Year :
- 2016
-
Abstract
- Background: Epitheliotrophic growth factors (GF) can be supplied topically to patients with severe keratopathy through a variety of blood-derived products. We compared GF content in adult peripheral blood serum (PB-S) and cord blood serum (CB-S) as potential sources of GF. To limit inter-individual variability the assessment was performed in maternal-child pairs at the time of delivery.<br />Material and Methods: The amounts of epidermal GF (EGF), insulin-like GF (IGF), transforming GF-beta (TGF-β), vascular endothelial GF (VEGF) in CB units collected from the umbilical vein and PB from mothers (each group n=30) were estimated by enzyme-linked immunosorbent assays. Obstetric characteristics and haematological data were recorded from the archives of the Emilia Romagna Cord Blood Bank. Statistical evaluations were performed by Wilcoxon's test and correlations between variables were determined using Spearman's (ρ) coefficient; p-values <0.05 were considered statistically significant.<br />Results: EGF, TGF-β and VEGF levels were significantly higher in CB-S than in PB-S (median 1,254.4 vs 646.0 pg/mL, 51.3 vs 38.4 μg/mL and 686.8 vs 30 pg/mL, respectively; all p<0.0001) whereas IGF content was significantly higher in PB-S than in CB-S (159.9 vs 53.5 pg/mL, respectively; p<0.0001). In CB-S, the CD34(+) cell concentration appeared to be related to EGF, IGF and TGF-β levels whereas white blood cell count appeared to be related to EGF and TGF-β levels. VEGF levels showed no relation to the haematological parameters considered. Platelet counts were not related to GF level in either CB or PB.<br />Discussion: The GF content in the two blood sources was different, with CB containing larger amounts. Each GF selectively regulates cellular processes involved in corneal healing, so the use of PB or CB should be targeted to supply specific GF on the basis of the type and severity of the keratopathy.
- Subjects :
- Adult
Female
Fetal Blood metabolism
Humans
Intercellular Signaling Peptides and Proteins chemistry
Intercellular Signaling Peptides and Proteins metabolism
Intercellular Signaling Peptides and Proteins therapeutic use
Ophthalmic Solutions chemistry
Ophthalmic Solutions therapeutic use
Serum metabolism
Fetal Blood chemistry
Intercellular Signaling Peptides and Proteins analysis
Keratitis
Ophthalmic Solutions analysis
Serum chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2385-2070
- Volume :
- 14
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Blood transfusion = Trasfusione del sangue
- Publication Type :
- Academic Journal
- Accession number :
- 26192781
- Full Text :
- https://doi.org/10.2450/2015.0020-15