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An improved method for the determination of 5-hydroxymethylfurfural in Shenfu injection by direct analysis in real time-quadrupole time-of-flight mass spectrometry.
- Source :
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Drug testing and analysis [Drug Test Anal] 2016 Jul; Vol. 8 (7), pp. 738-43. Date of Electronic Publication: 2015 Jul 22. - Publication Year :
- 2016
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Abstract
- The emergence of direct analysis in real time (DART) ion source provides the great possibility for rapid analysis of hazardous substance in drugs. DART mass spectrometry (DART-MS) enabled the conducting of a fast and non-contact analysis of various samples, including solid or liquid ones, without complex sample preparation or chromatographic separation. In this study, a modified DART-quadrupole time-of-flight mass spectrometry (DART-QTOF-MS) method was developed for identification and determination of 5-hydroxymethylfurfural (5-HMF) in Shenfu (SF) injection. The quantitative transfer of sample solution was introduced to the glass tips of DIP-it sampler at a fixed volume, which significantly increases the repeatability and accuracy of analytical results. The protonated ion of dibutyl phthalate in the atmosphere was used as the reference mass for TOF-MS recalibration during the data acquisition for constant high accuracy mass measurements. Finally, the developed DART-MS method was used to determine 5-HMF in seven batches of SF injection, and the contents of 5-HMF were not higher than 100 µg/mL. The results obtained were further confirmed by an ultra-high performance liquid chromatography combined with triple quadrupole mass spectrometer (UHPLC-QQQ-MS). The overall results demonstrated that the DART-QTOF-MS method could be applied as an alternative technique for rapid monitoring 5-HMF in herbal medicine injection. Copyright © 2015 John Wiley & Sons, Ltd.<br /> (Copyright © 2015 John Wiley & Sons, Ltd.)
- Subjects :
- Chromatography, High Pressure Liquid instrumentation
Drugs, Chinese Herbal administration & dosage
Equipment Design
Furaldehyde administration & dosage
Furaldehyde analysis
Injections
Limit of Detection
Mass Spectrometry instrumentation
Reproducibility of Results
Chromatography, High Pressure Liquid methods
Drugs, Chinese Herbal chemistry
Furaldehyde analogs & derivatives
Mass Spectrometry methods
Subjects
Details
- Language :
- English
- ISSN :
- 1942-7611
- Volume :
- 8
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Drug testing and analysis
- Publication Type :
- Academic Journal
- Accession number :
- 26197974
- Full Text :
- https://doi.org/10.1002/dta.1838